Need suggestion for stable cell line generation?

Hello All! I am trying to generate HCT 116 stable cell line. I studied many protocols and proceeded as follow: 1) I cultured cells in 6 wells plates at density of 5 x 10^5. 2) At 70% confluency,...

10 November 2016 9,838 4 View

PRPF4B (NM_003913) Human cDNA Clone?

05 June 2016 9,725 1 View

How to efficiently collect macrophages from mouse blood?

I am trying to collect macrophages from the mouse blood, but till now I am unable to do this perfectly. I feel following problems and need some recommendations: 1) Blood in mouse is very less,...

10 November 2015 9,538 2 View

How can I get the data from this western blot picture?

Hello everyone, As you can see in the picture, I have run two samples on SDS-Page, and signals have been detected by PARP antibody. Control has no band, while the treated sample is giving band,...

05 June 2015 9,134 8 View

Has anyone tried stable transfection of HCT-15 cells?

I am in search of recommendations about stable transfection of HCT-15 cells regarding selection of vector and antibiotic. It's my first time with this experiment and and I am searching a suitable...

05 June 2015 2,872 2 View

Why am I always getting dark background in western blot?

04 May 2015 5,864 9 View

How can one isolate cell membrane proteins from mammalian cells?

I would like to isolate cell membrane proteins from human colorectal cancer cells (HCT-15) and human leukemia cells (HL-60). I am in search of a suitable protocol. I have to order all the...

02 March 2015 7,606 8 View

Is Decursin Angelate available commercailly?

Hello All, I want to work with Decursin angelate, but i am not able to find it commercially by some company. Can any one tell me about its commercial availability?

02 March 2015 8,634 0 View

Did someone use "Prostaglandin E2 Express EIA KIT"?

01 February 2015 8,162 2 View

Does anybody know antibodies for single use?

01 February 2015 3,638 7 View

No title

02 March 2021 3,060 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View