Need suggestion for stable cell line generation?

Hello All! I am trying to generate HCT 116 stable cell line. I studied many protocols and proceeded as follow: 1) I cultured cells in 6 wells plates at density of 5 x 10^5. 2) At 70% confluency,...

10 November 2016 9,838 4 View

How to efficiently collect macrophages from mouse blood?

I am trying to collect macrophages from the mouse blood, but till now I am unable to do this perfectly. I feel following problems and need some recommendations: 1) Blood in mouse is very less,...

10 November 2015 9,538 2 View

How can I get the data from this western blot picture?

Hello everyone, As you can see in the picture, I have run two samples on SDS-Page, and signals have been detected by PARP antibody. Control has no band, while the treated sample is giving band,...

05 June 2015 9,134 8 View

Has anyone tried stable transfection of HCT-15 cells?

I am in search of recommendations about stable transfection of HCT-15 cells regarding selection of vector and antibiotic. It's my first time with this experiment and and I am searching a suitable...

05 June 2015 2,872 2 View

Why am I always getting dark background in western blot?

04 May 2015 5,864 9 View

How can one isolate cell membrane proteins from mammalian cells?

I would like to isolate cell membrane proteins from human colorectal cancer cells (HCT-15) and human leukemia cells (HL-60). I am in search of a suitable protocol. I have to order all the...

02 March 2015 7,606 8 View

Is Decursin Angelate available commercailly?

Hello All, I want to work with Decursin angelate, but i am not able to find it commercially by some company. Can any one tell me about its commercial availability?

02 March 2015 8,634 0 View

Does anyone have sample for Digital Data of Cell Cycle Histogram?

We know that flow cytometer giving us a histogram of cell cycle, uses some data from individual cells. Often these are FCS files. I mean that flow cytometer records the fluorescence of individual...

01 February 2015 7,555 2 View

Did someone use "Prostaglandin E2 Express EIA KIT"?

01 February 2015 8,162 2 View

Does anybody know antibodies for single use?

01 February 2015 3,638 7 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View