Hello All,
I want to work with Decursin angelate, but i am not able to find it commercially by some company. Can any one tell me about its commercial availability?
Hello All! I am trying to generate HCT 116 stable cell line. I studied many protocols and proceeded as follow: 1) I cultured cells in 6 wells plates at density of 5 x 10^5. 2) At 70% confluency,...
10 November 2016 9,949 4 View
Hello, I am going to generate a mammalian stable cell line, over expressed with PRPF4B. For this purpose I am looking for PRPF4B (NM_003913) Human cDNA Clone, in Neomycin/Hygromycin resistant...
05 June 2016 9,841 1 View
I am trying to collect macrophages from the mouse blood, but till now I am unable to do this perfectly. I feel following problems and need some recommendations: 1) Blood in mouse is very less,...
10 November 2015 9,653 2 View
Hello everyone, As you can see in the picture, I have run two samples on SDS-Page, and signals have been detected by PARP antibody. Control has no band, while the treated sample is giving band,...
05 June 2015 9,260 8 View
I am in search of recommendations about stable transfection of HCT-15 cells regarding selection of vector and antibiotic. It's my first time with this experiment and and I am searching a suitable...
05 June 2015 2,978 2 View
Please look at the attached photo. I have tried to develop MMP-3 band in HL-60 and HCT-15 cells. Usually i get the dark black backgroung, although i do the washing for about 1 h with 5 and 10...
04 May 2015 5,982 9 View
I would like to isolate cell membrane proteins from human colorectal cancer cells (HCT-15) and human leukemia cells (HL-60). I am in search of a suitable protocol. I have to order all the...
02 March 2015 7,709 8 View
We know that flow cytometer giving us a histogram of cell cycle, uses some data from individual cells. Often these are FCS files. I mean that flow cytometer records the fluorescence of individual...
01 February 2015 7,651 2 View
I am going to check the PGE2 secretion by using Prostaglandin E2 Express EIA KIT, cayman chemical. I am using it for the first time, so little hesitated. I have four samples, as below: (1) Control...
01 February 2015 8,282 2 View
Everybody knows that antibodies are so costly, ranging from 150-300 USD/antibody.These are available in 100-500 ul quantity. I want to test a cell signaling pathway, for which i need 5-10...
01 February 2015 3,738 7 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
Hi, I'm curious to know if data on chemical compounds from PubChem, such as water solubility properties, can be used to train a machine learning model for commercial purposes. Will this infringe...
30 July 2024 8,707 1 View
I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...
24 July 2024 8,696 0 View
Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...
19 July 2024 2,155 0 View
Dear Participants- Faculty and Researchers from Pakistani Higher Education Institutions, Thank you for participating in research study that aims to 'Analyze Individual and Institutional Factors...
18 July 2024 7,024 1 View
How to calculate percentage cumulative drug release when, Total volume of dissolution media = 250ml Added formulation was equivalent to 10mg of drug Sample withdrawn = 1ml each time Sink condition...
15 July 2024 4,192 1 View
Hi, In the 6 well plate, when I am seeding the cells. Do I add 1ml of complete media to one of the wells for the control at this stage or do I wait unit I am treating the plates to do that? Also...
13 July 2024 9,783 1 View