We are dealing with (gene expression/functional analysis) of metagenome isolated from hot spring water of gujarat, India. Can anyone suggest me cloning kit for cloning of metagenome (size >20 kb). We found various kits from literature survey like pGMET, Cosmid cloning kit (contains pWEB-TNC vector) etc. We suggest kit name which shows reproducible results with low cost, less time consuming experiment and efficient results.