Amitsinh Vijaysinh Mangrola

13 Questions 16 Answers 0 Followers

Questions related from Amitsinh Vijaysinh Mangrola

Does anybody know about transferring excel data into Paleontological Statistics Software Package (PAST)?

Hello, I am working on exploration of hidden microbial diversity using shotgun sequencing approach. I need to analyze my data using PAST. I installed PAST in my PC drive, I read the manual also,...

10 October 2015 4,693 5 View

Does anyone have idea regarding silver nanoparticle synthesis from plant water hyacinth (Eichhornia crassipes)?

We are dealing with the work entitled Synthesis of silver nanoparticle from plant. I have faced problem with particle size analysis by DLS. We got silver nanoparticle size in the micron range not...

07 July 2014 1,734 7 View

Does anyone have an idea regarding isolation of microorganisms from hot spring environments?

We are dealing with culturable analysis of microbial community found in hot springs. Which procedures should I apply for studying cultivable microbial diversity and how can we start our work in...

07 July 2014 8,801 6 View

Where can I get workshop to learn about NGS- ION TORRENT and NGS Data analysis by Bioinformatics tools?

We are dealing with microbial diversity analysis from different hot springs of Gujarat, India. I am interested to participate workshop of NGS-ION TORRENT and NGS Data analysis by Bioinformatics...

07 July 2014 4,919 3 View

Can anyone guide me the information about satellite colonies problem which occur during cloning?

After ligation, we got 50 colonies on L.B + Ampicillin plate, we select all colonies for gene expression study at flask level but we did not get expression at all. Recombinants are resisistant to...

07 July 2014 9,236 9 View

Does anyone know how to isolate Streptomyces hygroscopicus from soil for Sirolimus (Rapamycin) production at lab scale?

In our laboratory, we are stating research work on sirolimus production from natural isolates from soil and other sources. As per literature searching, high titre of sirolimus produced...

07 July 2014 8,645 2 View

Can someone provide information regarding growing MRSA strain in the laboratory?

I need information for in vitro cultivation of Methicillin resistant S.aureus strain which is isolated from hospital waste. Moreover can someone suggest appropriate methods for identification of...

06 June 2014 6,384 3 View

Does anyone have an idea regarding the selection of hypervariable regions of 16S rRNA gene for pyrosequencing?

Microbial diversity study.

06 June 2014 5,441 4 View

Which bioinformatic tool is good for comparative analysis of microbial diversity from hot spring water?

See above

06 June 2014 8,239 3 View

Which cloning kit is effective for cloning of large size DNA insert (>20 kb) extracted from hot spring water?

We are dealing with (gene expression/functional analysis) of metagenome isolated from hot spring water of gujarat, India. Can anyone suggest me cloning kit for cloning of metagenome (size >20...

06 June 2014 5,379 3 View

Does anyone have any ideas regarding the staining methods used for detection of small peptides on PAGE?

Study on protein purification

06 June 2014 3,201 3 View

Can someone let me know the procedure for biological synthesis of nanoparticles by bacteria?

I have isolated Bacillus licheniformis from hot spring water. We dealt with synthesis of nanoparticle from these bacteria. I did Nitrate reduction test and obtained positive results after 48 hrs...

06 June 2014 2,192 1 View

Does anybody know which Assembler should I use for NGS based Ion torrent data (size: 214 Mbp) assembly simply working in window?

Dear all, I am working on Hot spring metagenomics and we have Ion torrent data. The size of data: 214 Mbp. We want to upload assembled data for IMG/M ER. Now IMG accept only assembled data. Which...

01 January 1970 9,623 9 View