3 Questions 21 Answers 0 Followers
Questions related from Zhaowei Wu
Hey! Dear colleagues, I am designing my experiment. I want to know which nuclease can cut linear dsDNA but don't cut circular dsDNA? To be more specific. I am looking for a nuclease that cut the...
09 September 2016 852 3 View
Recently, I got problems about Pichia expression. My target gene was inserted into pPIC9K vector via EcoRI and NotI restriction sites. And then the SacI digested construct was transformed into...
03 March 2014 9,106 3 View
My target gene was inserted into pPIC9K and pPICZαA, respectively, and both were successfully verified by sequencing. Then constructs were digested with Sac1, extracted by ethanol precipitation,...
02 February 2014 7,915 18 View
