We want to isolate genomic DNA from plant leaves by using CTAB method.
Unfortunately we don't have PVP. Can we use other chemicals instead of PVP to absorb polyphenols? How can we modify the extraction method?
Actually i have not faced this type of problem. but in my opinion you have increase the amount of B-mecaptoethanol and the Chloroform:isoamyl alcohol thrice to remove polyphenols. try this may be helpful. gud luck
If you have sodium metabisulfite in your lab, that can do the trick for you. Its more potent than PVP in blocking polyphenolic compounds. hope this helps
regards
I really had problems with extracting RNA from elm trees.. My problem was polysaccharides but this protocol really helped.. Also applies for polyphenols it uses boric acid in the homogenization/extraction buffer.. Maybe you should also use Alcohol Precipitation not Isopropanol if you want DNA. In my samples DNA precipitated right after adding of Isopropanol....
'DNA extraction from plants: the use of pectinase' Dec 2001
Rogstad SH, Keane B, Keiffer CH, Hebard F, Sisco P,
_Plant Molecular Biol Reporter_19_(4):353
Perhaps this will help.
- Badges
- Science method