Why Slow freezing and Fast thawing in Cryopreservation of mammalian cells?

(a) What is the rationale of doing a slow freezing (controlled rate freezing)? (b) Why a quick thawing of frozen vials at 37 degree C? I read some research articles , but confused about...

11 December 2017 7,750 8 View

What defines the age of a primary cell line?

I am using some primary human cells for my experiments, But the activity/proliferation rate of the cells in their late passages (>P8) are very low and I can not use them for long like cell...

11 December 2017 4,809 5 View

What is the significance of Porosity evaluvation of injectable gels for tissue engineering application?

What is the relevance of studying morphology of an injectable gel? Can we visualize the porous structure of injectable gels by SEM imaging of freeze dried samples ? If not can you suggest me some...

04 May 2015 2,600 5 View

How to isolate fibrin from raw blood?

I am searching for a protocol to isolate fibrin from bovine blood.

10 November 2013 804 2 View

Can we store store 3M sodium acetate and ethanol added DNA extract at -20 Celsius for 3 days?

Usually we keep extract overnight after adding 1/10th volume of sodium acetate and 2 volumes of ethanol. My question is, if we keep it at -20 Celsius for 3 days, will it affect DNA quality? We are...

09 October 2013 1,009 15 View

Which chemical can replace the function of polyvinylpyrrolidone (PVP) in Plant Genomic DNA Extraction?

We want to isolate genomic DNA from plant leaves by using CTAB method. Unfortunately we don't have PVP. Can we use other chemicals instead of PVP to absorb polyphenols? How can we modify the...

08 September 2013 7,032 5 View

How to find the total length of CDS?

Using 'Highlight sequence features' option in NCBI, we have retrieved all CDS of a full genome. From this data set, CDS having less than 300 nucleotide were excluded. Now how should we proceed to...

08 September 2013 3,681 3 View

What is the difference between biotechnology and life sciences?

During my PG, in our university we had both life science and Biotechnology department. But both departments were teaching same subjects,doing similar research. I am confused between these two...

08 September 2013 7,691 12 View

What are the difference of Keratin from Human hair and chicken feather?

What are the differences of keratin molecule isolated from human hair and chicken feather in terms of structure and properties?

08 September 2013 4,781 1 View

How to prepare the sample for simple SDS PAGE of extracellular matrix derived from an adipose tissue?

In our lab we isolated the extracellular matrix from human adipose tissue and digested using 0.5M acetic acid. When I mixed the digested ECM with SDS loading dye (contains SDS, Bromophenol blue,...

07 August 2013 6,123 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View