Navjyoti Chakraborty

11 Questions 42 Answers 0 Followers

Questions related from Navjyoti Chakraborty

What kind of statistical analysis should be performed?

Suppose I have a wild type and mutants A, B and AB. I take control (untreated) as well as treat each of them (i.e the WT and the mutants A,B and AB) with some treatment/s and perform RT-qPCR for...

11 November 2014 5,086 9 View

What kind of statistical analysis should be carried out for RT-qPCR data, which has been used to validate microarray data?

Suppose I have around 20 differentially expressed genes for which RT-qPCR data has been generated, and the samples are a wild-type plant and a knock-out mutant. Which statistical analysis should...

10 October 2014 2,924 17 View

What is the maximum log2fold change detectable for repressed genes using qPCR?

We need to validate our microarray data for a few down-regulated genes which show log2FC values near -7. Can qPCR detect this level of down-regulation?

04 April 2014 3,506 11 View

Can anyone suggest a tool which can take AGI/TAIR ids as input and map them onto actual pathway maps?

See above

04 April 2014 5,681 2 View

Can someone suggest the controls for GST pull down?

I am attempting GST-pull down assay for finding unknown interacting partners using GST-fusion protein. Normally bait control, prey control, resin control are taken but I am planning to take also...

01 January 2014 9,974 3 View

Has anyone used the Xcelris plant RNA isolation minikit?

I have previously used the qiagen plant RNeasy minikit and that works very well, but I would like to know about the performance of this one.

12 December 2013 1,571 2 View

Nitrate estimation in plants?

Can anyone suggest a reliable method to estimate nitrate content in plant tissue other than kjeldahl method?

05 May 2013 6,015 8 View

Method/protocol needed

Does anyone have a protocol/suggestion on leaching out all forms of N from soil or a method to determine content of nitrate, nitrite or any other form of N present in soil?

03 March 2013 4,299 0 View

Expression of membrane protein

I am trying to express a plant membrane protein using pGEX vector in BL21 strain. I could express the protein but all went into the pellet fraction. Should I purify the protein from the pellet...

02 February 2013 3,132 14 View

Where can I find a protocol for Co-IP for protein-protein interaction?

I am planning to do co-IP for 1st time to find novel interacting partners. Need protocol for that along with all necessary precautions to be taken.

01 January 2013 4,125 2 View

Can anyone tell me if it's possible to modify mbSUS (mating based split ubiquitin system) to generate and screen cDNA library?

mbSUS has been used successfully by several researchers to screen for protein-protein interaction for membrane proteins but I could not find if anyone has used it to screen cDNA library. if it is...

11 November 2012 808 1 View