How to isolate fibrin from raw blood?

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Dr. Hussein Oleiwi Muttaleb Al-Dahmoshi

A rapid and simple procedure for the preparation, directly from clotted whole blood, of bovine fibrin free of cell membrane contaminants is described. The procedure exploits the ability of detergents to solubilize and extract cell membrane proteins from homogenized clot fragments. The non-denaturing detergents, TX-100 and DOC, used in sequence, or the denaturing detergent, SDS, used alone, proved equally effective. The resulting fibrin has the solubility properties and the subunit composition on SDS gels of highly cross-linked fibrin.

Nimal Raveendran

Thank you Dr.Hussein Al-Dahmoshi for your suggestion.

but the procedure is not clear for me, could you please elaborate procedure?