Help appreciated!! Thanks
Article Modification of the Cellular Antioxidant Activity (CAA) Assa...
Depending on your main objective, you can use any of these cell lines. As you may already know, HCT116 and Caco-2 are colon cancer cell lines while HepG2 is a liver cancer cell.
While many cell lines response differently, I suggest to use all of them and even other lines from other organs, because you are trying to show the antioxidant effect of your plant extract.
You can focus on a specific disease (such as colon cancer) and work on HCT-116 and Caco-2. Or just use large spectrum.
I have worked on both cell-lines (HCT-116 and caco-2) and I can say you can you use any of them, I say again depending on your aim. The options are unlimited.
Hi,
I had some difficulties to work with HCT116 for the study of oxidative stress, not in the growth and treatment, but in the application of my protocols for the measures because my cellular layers, fixed or not, were peeling off.
Why not consider the utilization several other cancer cell lines other than HCT116 and HepG2 ??
The referee of any journal would demand you to perform experiments using several cell lines!!
Thank you for the feedback Mr. Hichem. Since the elimination of antioxidant compound in liver is one of the major concern, can we say that liver cancer cell line is not a good model for antioxidant activity evaluation? If we take intestinal barrier as a important issue in this case, can we say that colon cancer cell line is more superior compared to Hepg2?
Thank you for the suggestion Mr. Yoshida. We are thinking of including Hs27 in our experiment as well.
Hi,
I think that the human hepatocellular carcinoma HepG2 usually used to evaluate the protective action of plant extract against ROS-induced oxidatative stress.
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