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Questions related from Go J Yoshida
Gain-of-functional mutant p53 exhibits a longer half-life period as compared with wild-type p53, which is why the significant accumulation of the mutant p53 in cancer cells is recognized in many...
28 April 2018 6,982 4 View
Besides high-resolution and ultra-high-field (7-tesla) magnetic resonance imaging (MRI),1 proton MR spectroscopy holds much promise for the diagnosis of multiple sclerosis (MS).2,3 1H-MR...
05 February 2018 3,241 5 View
There has been a growing concern about the significant shortage of the number of young physician-scientists, who can contribute to the development of basic medicine with the objective of...
30 January 2018 9,759 10 View
When you isolate endothelial cells of the blood vessels, using only CD31 antibody is not enough to isolate them? Why alpha-smooth muscle actin (alpha-SMA) antibody is necessary according to some...
28 January 2018 3,221 2 View
We have reported several years ago the 5-year-old male patient with Li-Fraumeni syndrome with osteosarcoma and atypical type of hepatic cancer. Intriguingly, the cancer stem cell marker CD44...
21 January 2018 7,872 2 View
The availability of magnetic resonance imaging (MRI) has led to an increase in the detection of abnormal brain findings even in cases when there are no clear neurological symptoms. When these...
16 January 2018 8,898 14 View
As described in the very recent case report by New England Journal of Medicine (Case 2-2017), the chelation therapy would increase the serum copper concentration and cause the worsening of...
20 January 2017 4,951 4 View
The emerging evidence suggests that the drawbacks of senescence are twofold. First, one might expect, senescence causes a loss of tissue repair capacity because of cell cycle arrest in progenitor...
19 January 2017 7,061 2 View
As indicated in the Case Record 23-2016 (July 28 issue) published by NEJM, a 46-year-old male patient developed rapidly progressive somnolence 12-14 hours after proximal femoral replacement...
20 August 2016 8,849 5 View
It is surprising in the following editorial that monocytes promote extravasation, which is the rate-limiting step of the distant metastasis! What about neutrophils!? Do they promote or suppress...
27 July 2016 6,243 6 View
Thiazolidinedione (TZD) binds to nuclear transcriptional factor PPAR-gamma, thereby causing metabolic reprogramming. This drug for the treatment for insulin resistance in type-II DM has been...
14 July 2016 4,675 2 View
TERT promoter mutations, IDH mutations and 1p/19q co-depletion are surely promising markers to classify clinically heterogeneous cases of glioma. Among Grade-IV gliomas patients, however, Figure1...
15 January 2016 7,666 1 View
Rasopathy is mainly composed of the cardiofaciocutaneous syndrome, Costello’s syndrome, Noonan’s syndrome, and the multiple lentigines syndrome. In addition, Legius syndrome is also well-known due...
15 January 2016 1,780 3 View
I am confused with the two kinds of cohort analyses composed of discovery cohort and validation cohort shown in Figure2 e-h in the attached file.According to ROC curves, both analysis tells that...
25 July 2015 9,328 6 View
It has been demonstrated that Notch negatively regulates the development of neuroendocrine tumor and small cell lung cancer since the master transcriptonal factors such as Hes1 and Hey1 inhibit...
24 July 2015 4,721 6 View
It has been reported that the significant decrease in hair follicle stem cell (HFSC) in the niche results in the formation of the cyst in the skin. However, I am quite confused how the cyst...
11 June 2015 2,527 3 View
I would like to perform Western blotting analysis of 3D-cultured cancer cells in collagen gel. In the case of Matrigel, Cell Recovery Solution (Corning) is very useful, but I do not know exactly...
12 May 2015 548 8 View
Perineural invasion is frequently observed in pancreatic cancers and they exhibit focal differentiation surrounding the peripheral nerves. Neighboring schwann cells lead to the differentiation of...
21 April 2015 5,415 2 View
Recent experiments revealed that both IR stress and DMBA treatment increase the stem cell factor (SCF) expression level at the secondary hair germ and dermal papillae. However, IR stress causes...
18 March 2015 3,063 2 View
We have previously identified the decreased expression of HFSC markers such as Lhx2, ColXVII, CD34 and Sox2 in the aging mice. However, the number of HFSCs was not so different as compared with...
03 February 2015 866 11 View
I often have difficulty in immunostaining of the structure of 3-D cultured cells, often referred to as spheroids or organoids embedded in Matrigel. What kind of methods/ protocols do you usually...
21 January 2015 4,674 9 View
We are interested in the dynamics of quiescent and proliferative cancer stem cells (CSCs) when tumor tissue shows invasion. We have previously investigated into the expression level of CD44...
18 January 2015 5,443 3 View
Bovine brucellosis is a major disease of cattle characterized by abortion during the last trimester of gestation. During many years important pieces of research have been done looking for a better...
01 January 1970 1,627 1 View
