Go J Yoshida

9 Questions 169 Answers 0 Followers

Questions related from Go J Yoshida

How to isolate only endothelial cells of blood vessels by flow cytometry?

When you isolate endothelial cells of the blood vessels, using only CD31 antibody is not enough to isolate them? Why alpha-smooth muscle actin (alpha-SMA) antibody is necessary according to some...

28 January 2018 3,199 2 View

How CD44 and Li-Fraumeni syndrome are related?

We have reported several years ago the 5-year-old male patient with Li-Fraumeni syndrome with osteosarcoma and atypical type of hepatic cancer. Intriguingly, the cancer stem cell marker CD44...

21 January 2018 7,851 2 View

What is the difference of BiP (GRP78) between normal and malignant cells in the regulation of ER stress??

Thiazolidinedione (TZD) binds to nuclear transcriptional factor PPAR-gamma, thereby causing metabolic reprogramming. This drug for the treatment for insulin resistance in type-II DM has been...

14 July 2016 4,652 2 View

Is metabolic reprogramming common in IDH-intact and triple-negative gliomas?

TERT promoter mutations, IDH mutations and 1p/19q co-depletion are surely promising markers to classify clinically heterogeneous cases of glioma. Among Grade-IV gliomas patients, however, Figure1...

15 January 2016 7,640 1 View

Why genetic syndromes associated with RAS mutations are rarely associated with the malignant neoplasms?

Rasopathy is mainly composed of the cardiofaciocutaneous syndrome, Costello’s syndrome, Noonan’s syndrome, and the multiple lentigines syndrome. In addition, Legius syndrome is also well-known due...

15 January 2016 1,770 3 View

Are there two kinds of cohort analyses?

I am confused with the two kinds of cohort analyses composed of discovery cohort and validation cohort shown in Figure2 e-h in the attached file.According to ROC curves, both analysis tells that...

25 July 2015 9,307 6 View

Does reduced expression of stem cell markers not necessarily mean the loss of stemness in hair follicle stem cells (HFSCs)?

We have previously identified the decreased expression of HFSC markers such as Lhx2, ColXVII, CD34 and Sox2 in the aging mice. However, the number of HFSCs was not so different as compared with...

03 February 2015 850 11 View

What is the most efficient method for IHC of spheroid/ organoids?

I often have difficulty in immunostaining of the structure of 3-D cultured cells, often referred to as spheroids or organoids embedded in Matrigel. What kind of methods/ protocols do you usually...

21 January 2015 4,661 9 View

Are dormant and proliferative cancer stem cells really enriched at the invasive front in the heterogeneous manner?

We are interested in the dynamics of quiescent and proliferative cancer stem cells (CSCs) when tumor tissue shows invasion. We have previously investigated into the expression level of CD44...

18 January 2015 5,420 3 View