Dear All,

I am working on research related to fabrication of glucose biosensor by using screen printed carbon electrodes (SPCE). I modified the working electrode with nanocomposite and then immobilised glucose oxidase-glutaraldehyde mixture on the modified SPCE.

Here are the detailed steps:

1. Preparation of nanocomposite.

2. Mix 20 μL of the 2% glutaraldehyde solution with 50 μL of Glucose oxidase. The mixture was allowed to sit for 30 minutes at room temperature before use.

3. Drop cast 3 μL of the nanocomposite onto the working electrode (WE) of SPCE.

Immobilization of Glucose oxidase-glutaraldehyde mixture:

1. Drop cast 3 μL of glucose oxidase-glutaraldehyde mixture on the modified SPCE

2. Allow the solution to adsorb onto the modified SPCE for 24 hours at room temperature.

3. After 24 hours, wash the unbound glucose oxidase from the working electrode using 0.01M PBS (pH 7).

4. Let the SPCE to dry at room temperature.

The MSDS mentioned that optimum storage temperature of glucose oxidase is -20°C.

As I am performing the immobilization of Glucose oxidase-glutaraldehyde mixture at room temperature for 24 hours, will it affect the stability of the glucose oxidase?

Plus, should I be storing the SPCE with immobilised glucose oxidase at room temperature or at cool temperature (probably 4°C)?

Help appreciated!! Thanks