We want to buy a flow cytometry for gene therapy and cell therapy purpose. I am looking forward to your expert opinion. Thank you.
Great question. Large part of the answer depends on your needs and what you plan to use it for the most. I wouldn't use something like an Aria (high end, expensive) as a simple analyzer, as it takes a fair amount of set up time and consumable, which aren't cheap.
How many parameters do you have in mind, more than 12, 14, 18, 20+? There's a post by Denis Baev back in 2018 here on Researchgate : "If you need no more than 13-14 parameter analysis in compact box try ACEA NovoCyte or AttuneNXT. For 15-18 colors the BD X20 or Cytoflex LS is a choice. Need 20+? Go with BioRad ZE5 or BD Symphony."
Also, are you thinking of using patient specimens for eventual therapy? If so, that means a number of certifications are likely required based on your institution/country/etc. If just using for in vitro diagnostics, this also requires certain certification(s). In the past, have had good luck with BD FACS Canto, BD in general has great customer support. Also, anyone in your group have FACs experience? Do you have a core facility to help? Important things to keep in mind, especially with more complex machines...
It depends on your experiment type. I found for cell therapy Flow Cytometer from BD is more accurate and user-friendly. Flowcytometer from Thermofisher is also good but it has blocking problem, Although they are cheaper than BD.
Hi Islam,
To answer your question will be important to know which flow cytometric pannel are you going to use for your experiment. I would suggest the BD FACS Canto as it is much simpler to use.
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