Has anybody administered MK-2206 (an orally active, allosteric inhibitor of AKT) intravenously to the mice?

We aim to use MK-2206 to inhibit AKT in mice. However, a large body of studies suggests that it should be administered orally. I was wondering if somebody could tell me that is it toxic for the...

07 February 2021 6,340 1 View

Could someone please suggest a suitable program for assembling plasmids containing repetitive sequences from short paired-end reads?

Hello! I'm trying to assemble some plasmid vectors that contain two repetitive regions (with around 500 and 250 bp) and it is being hard to get the complete sequence. If someone knows a better...

03 February 2021 6,379 3 View

Which are the appropriate solvents for n-hexane plant extract for in vivo animal models to avoid toxicity?n-hexane plant extract is not soluble?

My n hexane plant extract is not soluble in any solvent. I have tried water, normal saline, tween 20 (2%, 5%, 10%, 50%), tween 80 (2%, 5%, 10%, 50%), pure DMSO, ethanol (2%, 5%, 10%, 50%, 97%),...

16 January 2021 3,547 3 View

How to calculate the number of degrees of freedom in a Mantel test?

Hello, I've performed a Mantel test between my microbiological data and the geographic distance where those samples were collected, using a table with 516 samples. However, the test doesn't...

16 January 2021 8,713 4 View

What are the easy methods or techniques to synthesize green nano particles?

because many technical steps are there to get a particular plant nano particle and its expensive too

14 January 2021 1,556 4 View

What are the primary defects associated with welding druring construction-stages which might influence the capacity of the gusset-plate connections??

Dear researchers What are the primary defects associated with welding druring construction-stages which might influence the capacity of the gusset-plate connections in concentrated braced frames?...

06 January 2021 797 3 View

What is the new protocol followed by the World Health Organization to treat Covid 20?

The new strain of Covid 20 has spread in Britain, what is the new protocol for treating it, and is the old protocol followed by the World Health Organization suitable for treating the new strain...

21 December 2020 2,081 4 View

Women learning styles/ Women cognition development- Any suggestions for research based on Belenky's work?

I'm looking to expand my sources on the topic

10 December 2020 5,426 17 View

How can we convert powder (inorganic complex) in to a crystal?

I have powder (inorganic complex) and I want conversion it to crystal

03 December 2020 8,777 3 View

Can we use Google Form as our Research Tool?

I am confused regarding using Google Form as the questionaries tool for my study. It's effective during the covid-19 period but I didn't know if it is reliable or not. Please give your valuable...

18 November 2020 5,005 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View