Are SPL 96 well white plates autoclavable?

More Adriana Kapustová's questions See All

Could someone please suggest a suitable program for assembling plasmids containing repetitive sequences from short paired-end reads?

Hello! I'm trying to assemble some plasmid vectors that contain two repetitive regions (with around 500 and 250 bp) and it is being hard to get the complete sequence. If someone knows a better...

03 February 2021 6,379 3 View

How to calculate the number of degrees of freedom in a Mantel test?

Hello, I've performed a Mantel test between my microbiological data and the geographic distance where those samples were collected, using a table with 516 samples. However, the test doesn't...

16 January 2021 8,713 4 View

Which are the Non Small Cell Lung Cancer markers?

I would like to sort from a fresh specimen of lung cancer Non-Small Lung Cancer cells and cancer-associated fibroblasts to establish two different cell cultures. Which markers should I use? Thank...

04 January 2021 7,986 3 View

Women learning styles/ Women cognition development- Any suggestions for research based on Belenky's work?

I'm looking to expand my sources on the topic

10 December 2020 5,426 17 View

Sankey Diagram: Waht is the sample size needed?

Hello everyone. I would like to ask your help on the decision about the best methodology to determine the sample size to conduct an observational study. This is a study to be carried out by a...

16 September 2020 9,014 1 View

How to convert, excel files into (.vgd) or (.vms), ( .dts), (.dpr), (.vgl) files ?. Is there any software is available for this conversion?

I need a compatible file with Avantage for XPS of Themo Scientific but y only have excel files (.xls)

07 September 2020 215 3 View

Any expert on air pollution and its consequences on soil, plants and food production that is available to answer some questions?

Hello, I am a 3rd year Journalism student at Solent University. I am currently working on my Final Major Project. My project is about how air pollution influences our environment, and I will...

16 February 2020 6,332 2 View

I don't get a synthesis sample by pcr, what could I modify? considering that I already started from a standard protocol, I still don't get samples?

am carrying out a final point pcr from a standardized protocol, but I don't get results. I have modified concentrations of magnesium dichloride, DNTPS, DMSO, change the reaction water, the quality...

06 November 2019 7,767 2 View

In the sphere of education for the third millennium could education and inclusive school be an answer?

An inclusive education implies the development of inclusive societies. The inclusive, open-minded school for all educators and everyone, flexible and anchored in solving any educational problem...

07 March 2019 5,999 6 View

How to read the results from Illumina MiSeq machine?

Hi, everyone, I sequenced some samples in the Illumina MiSeq, got the results but now I don't know how to analyze them. I already read the User guide from Illumina, but it's only about the...

02 December 2018 9,374 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Malcolm Nobre

Hi Adriana Kapustová

SPL 96-well white plates (#30196) are not autoclavable.

SPL 96-well white plate (#30196) is made up of polystyrene material, and polystyrene is not autoclavable. These plates can be sterilized either by ethylene oxide gas sterilization or gamma irradiation.

If you are planning to re-use these plates, I suggest that you do not go ahead.

Best.

Adriana Kapustová

Malcolm Nobre thank you very much.