Which anti-GFP antibody do you recommend when using iDisco?

15 April 2019 0 9K Report

Hi all,

I'm planning to clear whole mouse hemispheres using iDisco, and would like to immunolabel endogenous GFP and mCherry. I got good recommendations on anti-RFP with rabbit as its host, so i'd like to use anit-GFP from another host- goat or guinea pig (rather not to use chicken). Does anybody have a good experience with a particular product in the iDisco protocol?

Thanks!

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