3D model in Cupcarbon, is it possible?

Dear colleagues, I need to create WSN model in 3 dimensions. I tried to use Cupcarbon for it but I couldn't find this function there. Could you explain me, how to do it in the package? Or to...

08 November 2018 1,481 1 View

What are your opinions about perspectives of Moebius strip like pathology diagnosis and treatment?

Proposed and tested an algorithm for diagnosis of Moebius strip like pathology, as prerequisite for treatment correction. The algorithm is reduced to initialization of study objects as a...

12 October 2018 5,854 3 View

fluorescent voltage sensors use?

Dear friends, i have a question regarding the fluorescent voltage sensors. I never tried it before, so I need good advice. What is your experience with Voltage dyes? If would use the...

11 January 2018 5,515 2 View

Do you have a job opportunity in Blockchain area?

Dear colleagues, My current project will finish this December and I'm looking for a new job opportunity in a Blockchain researching area. Write me please if you have an open positions for...

17 December 2017 4,894 3 View

How can I calculate using of energy by WSN node?

Could you write a link where I can find formulas for calculation an energy which is used by node of WSN (for transfer a data)?

17 December 2017 7,683 14 View

What problems are there for implementation blockchain technology in IoT, WSN and mobile devices?

All know that there are a problem POW for small devices. What with what problems have you encountered when implementing blockchain for small devices? What criteria do you use for evaluate new...

12 December 2017 9,647 6 View

What turbulence model and parameters to set on inlet of sintered/porous plate?

08 February 2017 4,939 3 View

Hello to everyone! Somebody knows where I can order Dermacentor reticulatus for laboratory culture?

I need a contacts!

15 September 2016 4,971 3 View

Is there a process of polynucleotide synthesis from nucleoside monophosphate, using energy from external chemical reaction?

Normally, DNA and RNA polymerization is realized by polymerases, using nucleoside triphosphates. The energy for the reaction is obtained from chopping off diphosphate group. I'm curious: is there...

15 December 2014 9,139 1 View

Can I make conclusions about certain protein redox states based on the redox state of cytoplasm?

Say, I have a protein in cytoplasm, which redox potential measured in vitro is +200 mV. The redox potential of cytoplasm appears to be say +50 eV. Knowing that, can I conclude that my protein is...

27 April 2014 516 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View