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Questions related from Sergii Pochekailov
I am studying massively parallel protein-protein interaction. Is there a good source for a ready DNA library, where a protein gene (or part of it) would be surrounded by standard adapters? Like...
06 July 2018 2,983 1 View
Typical function of aminoacyl tRNA synthetase (aaRS) is to attach amino acid to tRNA for following protein synthesis. I am curious, is there a natural process (or engineered aaRS), which would...
24 July 2015 3,780 2 View
Am I right that [2Fe-2S] adrenodoxin (or ferredoxin) molecule is reduced (and can carry) one electron at a time? I've read several papers claiming that Adx + 1e- --> Adx-but not Adx + 2e-...
10 March 2015 9,427 1 View
I'm trying to purify lentivirus packaging plasmids for future lentivirus production. I've heard the opinion, that simple maxi-prep yields DNA with endotoxins, which reduces the virus titration. To...
28 January 2015 328 5 View
Normally, DNA and RNA polymerization is realized by polymerases, using nucleoside triphosphates. The energy for the reaction is obtained from chopping off diphosphate group. I'm curious: is there...
16 December 2014 9,207 1 View
I'm trying to learn about phycobilin-related fluorescence. I've learned that there is a pathway of phycobilin biosynthesis, which consist of reduction of hemin through biliverdin to phycobilin...
25 November 2014 7,712 2 View
Say, I have a protein in cytoplasm, which redox potential measured in vitro is +200 mV. The redox potential of cytoplasm appears to be say +50 eV. Knowing that, can I conclude that my protein is...
28 April 2014 600 1 View
Say by screening the electron transport chain with reducing agents of different redox potential? Can you recommend what to read please?
28 April 2014 6,297 1 View
Does anybody know how to reduce/oxidize ferredoxin proteins, using non-natural agents (small molecules)? Which method is the best to use?
10 April 2013 459 2 View