Sergii Pochekailov

9 Questions 4 Answers 0 Followers

Questions related from Sergii Pochekailov

Where can I physically get a large library of protein genes?

I am studying massively parallel protein-protein interaction. Is there a good source for a ready DNA library, where a protein gene (or part of it) would be surrounded by standard adapters? Like...

06 July 2018 2,979 1 View

Can aminoacyl tRNA synthetase attach a polypeptide to tRNA instead of single amino acid?

Typical function of aminoacyl tRNA synthetase (aaRS) is to attach amino acid to tRNA for following protein synthesis. I am curious, is there a natural process (or engineered aaRS), which would...

24 July 2015 3,777 2 View

How many electrons are needed to reduce [2Fe-2S] adrenodoxin (ferredoxin)?

Am I right that [2Fe-2S] adrenodoxin (or ferredoxin) molecule is reduced (and can carry) one electron at a time? I've read several papers claiming that Adx + 1e- --> Adx-but not Adx + 2e-...

10 March 2015 9,424 1 View

Which method to use to purify lentivirus packaging plasmids? Will classic maxi-prep work?

I'm trying to purify lentivirus packaging plasmids for future lentivirus production. I've heard the opinion, that simple maxi-prep yields DNA with endotoxins, which reduces the virus titration. To...

28 January 2015 325 5 View

Is there a process of polynucleotide synthesis from nucleoside monophosphate, using energy from external chemical reaction?

Normally, DNA and RNA polymerization is realized by polymerases, using nucleoside triphosphates. The energy for the reaction is obtained from chopping off diphosphate group. I'm curious: is there...

16 December 2014 9,203 1 View

Does pure phycocyanobilin, not complexed with protein, exhibit any fluorescence?

I'm trying to learn about phycobilin-related fluorescence. I've learned that there is a pathway of phycobilin biosynthesis, which consist of reduction of hemin through biliverdin to phycobilin...

25 November 2014 7,709 2 View

Can I make conclusions about certain protein redox states based on the redox state of cytoplasm?

Say, I have a protein in cytoplasm, which redox potential measured in vitro is +200 mV. The redox potential of cytoplasm appears to be say +50 eV. Knowing that, can I conclude that my protein is...

28 April 2014 595 1 View

Is there any way to measure a redox potential of a certain protein in vivo?

Say by screening the electron transport chain with reducing agents of different redox potential? Can you recommend what to read please?

28 April 2014 6,294 1 View

How to oxidize/reduce adrenodoxin (or ferredoxin) with small molecules?

Does anybody know how to reduce/oxidize ferredoxin proteins, using non-natural agents (small molecules)? Which method is the best to use?

10 April 2013 456 2 View