11 Questions 4 Answers 0 Followers
Questions related from Sergii Pochekailov
I am studying massively parallel protein-protein interaction. Is there a good source for a ready DNA library, where a protein gene (or part of it) would be surrounded by standard adapters? Like...
06 July 2018 3,048 1 View
Typical function of aminoacyl tRNA synthetase (aaRS) is to attach amino acid to tRNA for following protein synthesis. I am curious, is there a natural process (or engineered aaRS), which would...
24 July 2015 3,814 2 View
The information I need is the amino acid sequence of Zn finger, the dissociation constant with its targeted DNA (or other measure of how strongly it binds) and the sequence of DNA target it binds...
25 March 2015 4,185 1 View
Am I right that [2Fe-2S] adrenodoxin (or ferredoxin) molecule is reduced (and can carry) one electron at a time? I've read several papers claiming that Adx + 1e- --> Adx-but not Adx + 2e-...
10 March 2015 9,486 1 View
I'm trying to purify lentivirus packaging plasmids for future lentivirus production. I've heard the opinion, that simple maxi-prep yields DNA with endotoxins, which reduces the virus titration. To...
28 January 2015 380 5 View
Normally, DNA and RNA polymerization is realized by polymerases, using nucleoside triphosphates. The energy for the reaction is obtained from chopping off diphosphate group. I'm curious: is there...
16 December 2014 9,264 1 View
I'm trying to learn about phycobilin-related fluorescence. I've learned that there is a pathway of phycobilin biosynthesis, which consist of reduction of hemin through biliverdin to phycobilin...
25 November 2014 7,762 2 View
Say, I have a protein in cytoplasm, which redox potential measured in vitro is +200 mV. The redox potential of cytoplasm appears to be say +50 eV. Knowing that, can I conclude that my protein is...
28 April 2014 659 1 View
Say by screening the electron transport chain with reducing agents of different redox potential? Can you recommend what to read please?
28 April 2014 6,341 1 View
Does anybody know how to reduce/oxidize ferredoxin proteins, using non-natural agents (small molecules)? Which method is the best to use?
10 April 2013 508 2 View
I need to link a protein to a DNA oligonucleotide. Protein must remain active. Linkage must be at a specific site (all proteins must be linked at the same site). How would you do that? Did anyone...
10 April 2013 7,158 2 View