3D model in Cupcarbon, is it possible?

Dear colleagues, I need to create WSN model in 3 dimensions. I tried to use Cupcarbon for it but I couldn't find this function there. Could you explain me, how to do it in the package? Or to...

08 November 2018 1,481 1 View

What are your opinions about perspectives of Moebius strip like pathology diagnosis and treatment?

Proposed and tested an algorithm for diagnosis of Moebius strip like pathology, as prerequisite for treatment correction. The algorithm is reduced to initialization of study objects as a...

12 October 2018 5,854 3 View

Where can I physically get a large library of protein genes?

I am studying massively parallel protein-protein interaction. Is there a good source for a ready DNA library, where a protein gene (or part of it) would be surrounded by standard adapters? Like...

05 July 2018 2,909 1 View

Do you have a job opportunity in Blockchain area?

Dear colleagues, My current project will finish this December and I'm looking for a new job opportunity in a Blockchain researching area. Write me please if you have an open positions for...

17 December 2017 4,894 3 View

How can I calculate using of energy by WSN node?

Could you write a link where I can find formulas for calculation an energy which is used by node of WSN (for transfer a data)?

17 December 2017 7,683 14 View

What problems are there for implementation blockchain technology in IoT, WSN and mobile devices?

All know that there are a problem POW for small devices. What with what problems have you encountered when implementing blockchain for small devices? What criteria do you use for evaluate new...

12 December 2017 9,647 6 View

What turbulence model and parameters to set on inlet of sintered/porous plate?

08 February 2017 4,939 3 View

Hello to everyone! Somebody knows where I can order Dermacentor reticulatus for laboratory culture?

I need a contacts!

15 September 2016 4,971 3 View

Is there a process of polynucleotide synthesis from nucleoside monophosphate, using energy from external chemical reaction?

Normally, DNA and RNA polymerization is realized by polymerases, using nucleoside triphosphates. The energy for the reaction is obtained from chopping off diphosphate group. I'm curious: is there...

15 December 2014 9,139 1 View

Can I make conclusions about certain protein redox states based on the redox state of cytoplasm?

Say, I have a protein in cytoplasm, which redox potential measured in vitro is +200 mV. The redox potential of cytoplasm appears to be say +50 eV. Knowing that, can I conclude that my protein is...

27 April 2014 516 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Open Source IoT Simulators supported by Machine Learning?

Dear Research Gaters, Let me first add context to the question. I am looking to simulate the waste management system and resources of a city. The hardware devices I need to simulate include,...

21 February 2021 2,333 4 View