Hi everyone,
I'm looking for a supplier of synthetic miRNA (lyophilized) which I can use as spike-in control. I would like to order cel-miR-39 and cel-miR-54.
Would be great if someone can help me out.
Regards
Felix
Hi!
I'm generally using LNA technology. If you want that specific mir you can find it in the Exiqon website http://www.exiqon.com/rna-spike-in-kit
I think they are the best to work with mir..anyway it works in Syber green, so if you're using TaQman it doesn't fit!
You can order cel-miR-39 at Qiagen.
Exiqon is the best place to go...
I am planning to use the Exicon's kits for isolation , RT and qPCR, but I want to use a cel-miR-39 spike in RNA from Qiagen in combination with a LNA primers. Did anyone use that combination or read about it in some publication?
Is there any difference between mimic mirna and synthetic mirna?
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