Sheyla Mayumi Kuniwake

4 Questions 6 Answers 0 Followers

Questions related from Sheyla Mayumi Kuniwake

Volcano plot analysis - any recommendations?

I need a free and easy-to-use software to perform volcano plot analysis with my RT-qPCR results. What is your recommendation?

02 February 2014 3,667 4 View

Problems with purity and amount of extracted RNA

I used miRNeasy Serum/Plasma kit (Qiagen) to purify RNA from 5 serum samples (200 ul), as indicated in the protocol, and then I quantified with the spectrophotometer NanoVue. No sample had a good...

09 September 2013 6,012 13 View

Volume vs. Amount for RT

I am using miRNeasy Serum/Plasma kit (Qiagen) to purify total RNA and I detected a low RNA concentration and low purity (260/280) using NanoVue. Bioanalyzer is the most recommended strategy to...

08 August 2013 6,396 2 View

Is there a difference between the terms "overexpression" and "upregulation"?

These terms are a little confusing to me. There are some articles that refer to them synonymously, but I'm not sure if that's correct. If they are different, can someone explain me what are these...

12 December 2012 8,681 5 View