Who has experience with housekeeping genes as reference for qRT-PCR for samples from muscle tissue?

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Elisabeth Buchberger

You cold also use 36B4 (acidic ribosomal phosphoprotein)

Works nicely and is stable.

NM_001002.3; NM_053275.3

FWD: CGCACCGCCGTGATG

REV: AATAGTTGGATGATCTTAAGGAAGTAGTTG

PROBE: AAGACAGGGCGACCTG

Felix George

Thanks for your answer. I'll think about to use it for my investigations with miRNA. The sequence is short so maybe it is useful.

EDIT: DId you work with mice or rats?

No. Human blood samples and cell culture.