Ashok Singh

2 Questions 5 Answers 0 Followers

Questions related from Ashok Singh

Why do we see a fold change differences greater in microRNA profiling compared to SYBR Green based real time PCR validation?

Recently, I am validating some of the microRNA found to be deferentially expressed in one of my study through microRNA profiling. That mouse microRNA is 2 fold change compared to my control...

11 November 2013 3,417 2 View

Why do we dilute the cDNA for Real time expression studies of microRNA expression ?

I want to know why we dilute the cDNA for Real time expression studies of microRNA expression.

10 October 2013 8,168 0 View