How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

Can Scree Plot in SPSS show an incorrect data on X-axis?

Hi! I'm working on a PCA in SPSS 23. I have 78 items. PCA extracts 16 components. However, scree diagram is plotted with items (manifest variables) on the X-axis. Not components (factors). See...

27 January 2021 5,919 3 View

How to measure Tg (Glass Transition) in lyophilised sugar/protein matrices using DSC?

I have been attempting to measure the glass transition temperature of various amorphous powders consisting of sugar/protein mixtures and have had little luck. I typically use a scanning rate of...

13 January 2021 10,022 3 View

Where to publish my methodological working paper?

Recently I decided to switch my PhD project to a cumulative form. I have already written an extensive methodological chapter about my adaptation of interpretivist methodology and ethnographical...

11 January 2021 5,337 6 View

Why does reducing my protein samples with TCEP cause the emergence of a new, much lower MW band in SDS PAGE?

Hi! I study the effect of formulation excipients on protein degradation pathways and was looking disulfide-mediated aggregation by comparing reduced/non-reduced SDS page samples. I am using TCEP...

17 December 2020 2,724 3 View

How can I add details or change information to my article?

I missed to add all coauther to my publication. How can I add them??

10 December 2020 8,785 3 View

Do you know of a test dataset of research papers in computer science field?

Is there a freely available test dataset of research papers in computer science field? I need to evaluate my text retrieval system which uses a computer science domain ontology, so the dataset...

19 October 2020 5,412 3 View

Oxygen detection using Ru(bpy)?

I am trying to detect if my nanoparticles generate oxygen. Is there a way I can use Ru(bpy) and obtain an emission spectra? (Any other "quick and fast" method I can use to detect oxygen generation?)

26 August 2020 1,942 3 View

What are some in vitro experiments that can be performed to target hypoxia?

I want to show that my developed nanoparticle targets hypoxia but from doing research it seems like most hypoxic targeting related experiments are done in vivo. I'd like to be doing in-vitro...

16 August 2020 3,695 4 View

Can anyone help me understand this ROS protocol using CM-H2DCFDA dye?

Hi, this is my first time trying this ROS experiment and I am not a biologist or familiar at all with cell stuff. So I would appreciate if anyone can break down this procedure step by step; that I...

08 August 2020 6,857 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View