Using 16 pb primers in PCR, and the Electrophoresis gel displayed a size of the PCR products: How can I find size of Allele?

if the estimate size of the allele is 245-320bp. What is the accurate method to find the size of the alleles? Thank you very much for the help

06 July 2015 4,788 2 View

Do forward and reverse primers hybridize as a whole quantity, or some of their complementary nucleotides to the DNA template?

02 March 2015 851 0 View

What is the formula to calculate Tm of primers F: TCAAAGGAATGTTGGG and R: ACGCACATAGGGATTG to use in PCR?

02 March 2015 9,414 8 View

Is anyone familiar with the Ym30 pair primers?

09 October 2014 5,359 1 View

What is the role of MgCl2 in PCR?

PCR amplification effects by the MgCl2 concentration. That what happen in an experiments I carried out. I need more advices and recommendation until what concentration the amplification could...

08 September 2014 7,192 12 View

I have a problem with tailed primers. The forward primer is cut and only M13 left. Can anyone help?

I am using M13 tailed primers with the forward which initially starts the amplification PCR reaction. When the part of the Forward Primer is cut off, can the M13 tailed primer amplify later on...

08 September 2014 2,533 3 View

What are some methods for DNA Pooling (grouping)?

When putting together more than one sample of DNA in order to run the PCR gradient, what are the methods to group different samples of same species of DNA together besides having the Nano Drop...

04 May 2014 2,603 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View