Aldosky Shaalan

8 Questions 5 Answers 0 Followers

Questions related from Aldosky Shaalan

Using 16 pb primers in PCR, and the Electrophoresis gel displayed a size of the PCR products: How can I find size of Allele?

if the estimate size of the allele is 245-320bp. What is the accurate method to find the size of the alleles? Thank you very much for the help

07 July 2015 4,833 2 View

Do forward and reverse primers hybridize as a whole quantity, or some of their complementary nucleotides to the DNA template?

In PCR, the two primers hybridize to the DNA target. Forward primers anneals to the DNA target at the 3' toward 5' terminus where the 3 carbon bonds by a covalent hydroxyl bond with the phosphates...

03 March 2015 892 0 View

What is the formula to calculate Tm of primers F: TCAAAGGAATGTTGGG and R: ACGCACATAGGGATTG to use in PCR?

Can I use this rule of Melting Temperature = 4(G+C) +2(A+T) “Wallace rule or there is a better formula to calculate Tm. I am using this buffer 10X ViBuffer A (without MgCl2} 500mM KCl, 100mM...

03 March 2015 9,460 8 View

Is anyone familiar with the Ym30 pair primers?

If I integrated M13-SSR (5ʹ-CACGACGTTGTAAAACGAC-3ʹ) within the forward primers of the Ym30 pair primers which is F: CACGACGTTGTAAAAC R: CACGTATTAACTCCAT . Can I write the new integration of the...

10 October 2014 5,442 1 View

I have a problem with tailed primers. The forward primer is cut and only M13 left. Can anyone help?

I am using M13 tailed primers with the forward which initially starts the amplification PCR reaction. When the part of the Forward Primer is cut off, can the M13 tailed primer amplify later on...

09 September 2014 2,572 3 View

What is the role of MgCl2 in PCR?

PCR amplification effects by the MgCl2 concentration. That what happen in an experiments I carried out. I need more advices and recommendation until what concentration the amplification could...

09 September 2014 7,242 12 View

What are some methods for DNA Pooling (grouping)?

When putting together more than one sample of DNA in order to run the PCR gradient, what are the methods to group different samples of same species of DNA together besides having the Nano Drop...

05 May 2014 2,641 1 View

Where can I get Malayisan Yam samples?

I am student at Nottingham University, working on a project on Yam in Malaysia. I am having difficulty finding where to collect samples, because I am an international student. I would be very...

05 May 2014 8,912 1 View