Where can I find the following paper: "In vitro differentiation of transplantable neural precursors from human embryonic stem cells"?

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Is there a way to conduct EFA for two groups (e.g., immigrant descent/non-immigrant descent)?

I am asking this for a scale development study. We have mutual/same items asked to both groups, and some items are asked only to a specific group (e.g., people of immigrant descent). Is there a...

21 May 2024 7,032 1 View

Is there any gene in introns related with phenotype changes of breeds?

I wonder is there any information about the mutation in intron regions related to phenotype.

11 December 2021 4,501 10 View

I will study the phosphorylated STAT3 in synovial tissue by western blotting but i can't same day. How can i keep tissue until the day i work?

When should i add protease and phosphatase inhibitors?

06 May 2021 5,302 4 View

What are the features and advantages of the random effect ordered panel probit model over the fixed effect ordered panel logit model?

As it is known, the application area of ordered discrete choice models is quite wide. So why is logit model common in fixed effect models, whereas probit model is used in random effect models?...

15 April 2021 4,983 4 View

How to estimate interrater-reliability of a variable with 3 columns of answer?

Hello everyone, In my study, I asked participants to write 3 different memories. After collecting data, 2 raters coded these memories. Now I have 3 columns for the first rater and 3 columns for...

06 February 2021 7,791 8 View

Ion exchange vs Size exclusion chromatography based on yield and purify?

What is differences between ion exchange and size exclusion chromatography based on yield and purify. Which one has higher purity/yield and reliable?

18 April 2020 4,193 5 View

Can anyone recommend a good protocol for vesicle isolation from cultured neurons?

Dear all, I am currently trying to isolate synaptic vesicles from iPSCs derived cortical neurons. As you can imagine, I do not have massive number of cells, max I would guess, I have 10 million...

08 April 2020 3,908 0 View

Can anyone recomment a paper or explain the working principles of the multi-channel electrode assay (MEA, Multi Channel System) for neuronal activity?

I was unfortunate to find a paper where the working principle of the MEA technology is explained. How does it do the measurements? How the electrodes getting the signal and convert it to voltage?...

07 February 2020 1,240 1 View

Anybody use CTD-Diver?

If you are using CTD-Diver, can you tell me which brand and what are the advantages and disadvantages of your equipment?

07 July 2019 9,828 0 View

Does chloroform is avaliable for melatonin solution and appropiate concentration?

I am looking for melatonin solvent, particularly chloroform,.I found DMF and DMSO concentration but I did not see for chloroform.

19 July 2018 4,476 1 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View