Tuğçe Munise Şatır

6 Questions 18 Answers 0 Followers

Questions related from Tuğçe Munise Şatır

Can anyone recomment a paper or explain the working principles of the multi-channel electrode assay (MEA, Multi Channel System) for neuronal activity?

I was unfortunate to find a paper where the working principle of the MEA technology is explained. How does it do the measurements? How the electrodes getting the signal and convert it to voltage?...

08 February 2020 1,083 1 View

How to perform a PCR reaction on PFA fixed tissue whole zebra fish embryo??

Dear Colleagues, I am currently having difficulties with my PCR reaction from pre- stained PFA fixed zebrafish embryos. I had to stained the embryos and then needed to perform a PCR reaction...

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IPSCs culturing problem,Cells are not attaching!

Hi everybody, We are struggling with a huge problem since a long time in our cell culture. Although we culture the our iPS cell (E8 and its supplement with Geltrex Coating) according to the same...

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Any advice using mitotic inhibitor during neuronal differentiation from iPSCs?

We have been following protocol which explains how to obtain cortical neuron from iPSCs. Despite having really promising results, we have mixed culture, so far. I am wondering if I can use mitotic...

17 March 2015 5,074 6 View

Could anybody help me to find the best vector in order to get transient transfection into the human fibroblast?

I would like to perform transient transfection into the human primary fibroblast with gata4 mef2c and tbx5. I do not want to perform viral transfection. Could you give me some suggestions?

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Is there any possibility that Oct4 can be detected in human skin fibroblast cells nuclei by immunostaining?

I used to fibroblast as a negative control for Oct4 immnostaining but I observed that my fibroblast was stained by Oct4 primary antibody. I also used to the other negative control with only...

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