Tuğçe Munise Şatır

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Questions related from Tuğçe Munise Şatır

Can anyone recommend a good protocol for vesicle isolation from cultured neurons?

Dear all, I am currently trying to isolate synaptic vesicles from iPSCs derived cortical neurons. As you can imagine, I do not have massive number of cells, max I would guess, I have 10 million...

09 April 2020 3,879 0 View

Can anyone recomment a paper or explain the working principles of the multi-channel electrode assay (MEA, Multi Channel System) for neuronal activity?

I was unfortunate to find a paper where the working principle of the MEA technology is explained. How does it do the measurements? How the electrodes getting the signal and convert it to voltage?...

08 February 2020 1,100 1 View

How to perform a PCR reaction on PFA fixed tissue whole zebra fish embryo??

Dear Colleagues, I am currently having difficulties with my PCR reaction from pre- stained PFA fixed zebrafish embryos. I had to stained the embryos and then needed to perform a PCR reaction...

03 April 2018 1,994 3 View

IPSCs culturing problem,Cells are not attaching!

Hi everybody, We are struggling with a huge problem since a long time in our cell culture. Although we culture the our iPS cell (E8 and its supplement with Geltrex Coating) according to the same...

28 June 2017 8,460 24 View

Has anybody tried to perform APP knockdown via CRISPR?

My plan is to perform the CRISPR onto iPS cells. I want to have APP knockdown iPS cell line and differentiate them into the neuron. I do not know whereto start. I plan to order ready vector for...

18 September 2015 7,912 1 View

Any advice using mitotic inhibitor during neuronal differentiation from iPSCs?

We have been following protocol which explains how to obtain cortical neuron from iPSCs. Despite having really promising results, we have mixed culture, so far. I am wondering if I can use mitotic...

17 March 2015 5,079 6 View

Could anybody help me to find the best vector in order to get transient transfection into the human fibroblast?

I would like to perform transient transfection into the human primary fibroblast with gata4 mef2c and tbx5. I do not want to perform viral transfection. Could you give me some suggestions?

18 September 2014 9,331 4 View

When I get Oct4 and Tra-1-60 positive staining with a reprogrammed cells even if they don't make a colony, can I say I have an iPS?

I am trying to obtain iPS cells through feeder and virus free condition. My cells stained with Oct4 and Tra-1-60 but they didn't make a colony. Their shape doesn't seem as a fibroblast but also...

16 July 2014 9,338 10 View

Is there any possibility that Oct4 can be detected in human skin fibroblast cells nuclei by immunostaining?

I used to fibroblast as a negative control for Oct4 immnostaining but I observed that my fibroblast was stained by Oct4 primary antibody. I also used to the other negative control with only...

14 July 2014 5,817 8 View