Dear all, I am currently trying to isolate synaptic vesicles from iPSCs derived cortical neurons. As you can imagine, I do not have massive number of cells, max I would guess, I have 10 million cells in total. Most of the protocols out there is for whole brain, which will have much more cells than I can reach.
The other question is that, I would also like to isolate exosomes from the cell conditioned media. From the same cells that I would isolate the synaptic vesicles. As you can imagine not many method regarding this either.
Could anyone of you recommend some methods or share their own?
Thank you very much for your help in advance.
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