Hello

Snap freeze the tissue in liquid nitrogen and store the tissue in

-80 degree C until further use. But on the day when you thaw the tissue immediately place the tissue in lysis buffer containing protease and phosphatase inhibitors because thawing can degrade your protein of interest.

Good Luck.

Hello, thank you so much :) Malcolm Nobre

Is it enough to put tissue in cyro tubes and freeze it? Or should we freeze it in PBS? Malcolm Nobre

Hello Tuğçe Yavaş

It is enough to put the tissue in cryo tubes and freeze.