09 March 2017 2 5K Report

How can i procure the TB LAMP KIT by EIKEN for diagnosis of pulmonary tuberculosis ?

More Vinaykumar Hallur's questions See All
For Plant protein Extraction instead of Liquid N2 can i use Glass beads?

During total Plant Protein extraction commonly use Liquid N2 for removal of moisture from Plant parts, instead of using Liquid N2 can I use Glass beads for protein extraction, is it have any bad...

25 November 2021 8,866 2 View

Has anyone used genomic DNA for Recombinase Polymerase Amplification Primer Screen ?

TwistDx the company which sells the TwistDx kits for RPA. It recommends using 40 copies of the target sequence for a primer screen. I understand 40 copies can be made by using Clone. Has anyone...

07 May 2021 2,631 0 View

How can I install Rpy2 in my system with out having the below mentioned errors?

We are currently working on a project which requires us to wrap the r codes into python notebook, for which we preferred rpy2 package. Initially it was working fine, but recently we came across...

25 November 2020 10,034 2 View

Do we need to follow CPCSEA guidelines when using insect models for our research?

Since insects are invertebrates what are the guidelines for handling them in research in India ?

22 September 2018 4,581 2 View

Is there any way of confirming whether a fungus has sporulated without making a tease/tape mount??

I am having a black fungus which is growing very slowly and I am scared that I will contaminate the culture of I open the plate for preparing a tease mount

01 November 2017 7,867 2 View

What has more clinical significance Helicobacter pylori MLST or virulence genotyping ?

I have read that Helicobacter pylori MLST is more meaningful in understanding  human migrations rather than helping in understanding the clinical importance of a particular population of...

10 February 2017 3,799 3 View

Why cant we use Bst polymerase(used for LAMP) in place of taq polymerase for PCR ?

I was just thinking why we could not replace the taq polymerase with Bst polymerase used in LAMP and run our PCR ?

28 November 2016 2,744 4 View

Can any one please let me know the informations regarding metal matrix composite of AL 6061+ ZIRCONIUM+ FLY ASH?

i am working on the project of study of machinability of Alluminium 6061, zirconium, fly ash composites. before i take a step ahead i want to get some details about how the results of this...

25 July 2016 9,886 2 View

Join to Mechanical Engineers

I want to join this team

06 April 2011 3,101 1 View

HAs anyone used the new capillary microculture method for culturinh Helicobacter pylori?

Has anyone used the capillary microculture method for isolating Helicobacter pylori from gastric biopsy ? Please have a look at the link below ?

01 January 1970 4,372 3 View

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When Using SYBR Green for Flourescent Detection of LAMP Products what causes an Overshoot in Flourescence Reading?

The Graph below shows an example of an amplification curve that shows an overshoot in fluorescence. I am using SYBR Green, BST polymerase, and 6 LAMP primers in the mixture and amplifying at 65...

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Unification of General Relativity and Quantum Mechanics is possible ?

How can anyone develop a theoretical framework that successfully unifies general relativity and quantum mechanics? how this new theory should accurately describe gravitational interactions at...

10 July 2024 5,040 8 View

When using the relative quantification method in the 7500 PCR system, is a reference sample essential?

Hello. In our laboratory, we are currently conducting Loop-Mediated Isothermal Amplification (LAMP) to detect insert DNA in plasmid vectors. When using the LAMP method, we typically use 8 tubes,...

24 June 2024 645 1 View

What could be the reason for PCR amplification failure using Whole genome amplification products??

I have designed primers for HBB Gene and the template is Whole Genome amplification(WGA) products. The primers work in some samples and in some they don't. I measured the OD of both the samples....

23 June 2024 3,362 2 View

Title: Single Band in Positive Control LAMP Assay - How to Fix?

I am conducting a LAMP assay, and while the negative control shows no bands as expected, my positive control samples consistently display only a single band instead of the anticipated ladder-like...

18 May 2024 4,853 3 View

What is the reason for the color of the Agrobacterium cell pellet? Is it a normal cell color, or could it indicate contamination?

Strain: LBA4404 Growing media: YEB (pH 7) Temperature: 28°C, 200 rpm (overnight culture) Antibiotic use: Kanamycin and Streptomycin

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Could someone explain me how to design an ITC experiment to get a result as quickly as possible?

Hi there, My area of research is an aqueous Zinc ion battery and my goal is to find the binding affinity and thermodynamic properties of Zn-ions binding with polyacrylic acid (PAA) by ITC...

30 April 2024 4,171 0 View

Is there any Research article that demonstrate 'Effect of Primer Melting temperature on LAMP amplification'?

I am looking for any research article that demonstrate the effect of different length and Tm of Primers used in LAMP reactions. Does the Bst 2.0 Polymerase have any impact on the Tm of primers?

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Itc experiments procedure?

I'm a beginner in ITC, does anyone have an idea why when measuring the heat of dilution and in measuring the interaction of HSA with the drug or even with the buffer itself, the curve goes up? HSA...

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How to fit the complex ITC data?

I've done the ITC experiments to find the calcium-binding affinity of my protein. I used the built-in program, the MicroCal-ITC analysis software to fit the data. The mode that I used to fit is...

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