During total Plant Protein extraction commonly use Liquid N2 for removal of moisture from Plant parts, instead of using Liquid N2 can I use Glass beads for protein extraction, is it have any bad impact on the Total protein of the plant
I wouldn’t recommend changing N2 because it causes cell lysis and solvent penetration easier and it also prevents degradation. So I would say that if you change to the glass beads it would change the final protein concentration as well because the solvent did not achieved its best potential