What is the reason for the color of the Agrobacterium cell pellet? Is it a normal cell color, or could it indicate contamination?

More Rudra Prasad Khuntia's questions See All

How activation energy varies with temperature for second order phase transition?

how activation energy varies with temperature for second order phase transition. please share some references.

25 April 2024 4,814 1 View

Arruda-Boyce(8-chain model) UMAT availability?

Can someone provide Arruda-Boyce(8-chain model) UMAT code for Abaqus?

12 March 2024 2,854 0 View

Is there any subroutine in Abaqus that I can use to delete the whole part?

I can delete the elements, but can we also delete the part when the strain in an element of the part exceeds some value?

06 March 2024 1,245 3 View

Can we extract and use only the sequences from the aligned regions for constructing phylogenetic tree and delete the unaligned sequences?

Suppose we are performing a phylogenetic analysis among different fungal species and we wanted to see how closely our species of interest is related with the other species across different...

28 January 2024 9,895 4 View

How to increase bootstrapping values of a phylogenetic tree?

I want to establish a phylogenetic relationship of our fungal species of interest along with the other fungal species belonging to the same order (Hypocreales). In order to perform this, we have...

22 January 2024 9,174 5 View

How to use S8 parameter for PW91 functional in Gaussian16 properly?

Hello everyone! I am trying to obtain simple geometry optimization of a single DNA-PNA mononucleotide having around 80 atoms using Gaussian16 This is the main input line I am using. #n...

01 November 2023 5,356 0 View

Sir, how can we draw a 3D surface by rotating an analytical curve about X-axis in CST MW Studio?

The equation of the analytical curve is :u(t)=t & v(t)= sqrt((R^2)-(t^2))-B; where R=2.125 & B=1.875, t=0 to 1. I was able to draw the analytical curve..but wasn't able to rotate it about...

15 December 2022 8,293 0 View

How to get the graph of shear stress and normal stress in PFC 2D?

I'm performing a direct shear test in PFC2D. I'm able to get the graph of normal stress vs no. of steps and shear stress vs no. of steps. But, I'm finding it difficult to get normal stress vs...

28 October 2021 8,844 3 View

What would happen when using single isobaric tag for different treatments in peptide analysis experiment ?

I have two treatments; resistant and susceptible. I have to use them in control experiments with replications. How I can use iTRAQ based different labelling to those different treatments’s...

24 October 2021 5,311 3 View

How it is possible that DNA primers previously working suddenly stop to worked?

I have used the same exact sequences in primers while ordering the new primers. The old primers are that I used previously which was worked successfully. Similarly, the sequences in tubes are also...

20 August 2020 3,324 12 View

Is skin yellowness an numerical or ordinal variable?

I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...

11 August 2024 4,793 1 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 806 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View