How it is possible that DNA primers previously working suddenly stop to worked?

I have used the same exact sequences in primers while ordering the new primers. The old primers are that I used previously which was worked successfully. Similarly, the sequences in tubes are also...

20 August 2020 3,132 12 View

How to perform Reciprocal Space Mapping (Symmetric and Asymmetric) XRD for oriented thin films?

Let's suppose I have an STO substrate and I have deposited a thin film of another material and it is highly oriented and STO as well as my material both form are cubic. To better understand the...

14 May 2020 6,359 3 View

What is the range of maximum DC link voltage on the grid connected solar system at present? Why we need to go for higher DC voltage?

What is the range of maximum DC link voltage on the grid connected solar system at present? Why we need to go for higher DC voltage?

19 April 2017 7,550 3 View

Is this possible to compute Raman spectra in Gaussian for BaTiO3 type perovskite oxides?

I want to calculate DOS, Band structure, IR spectra and Raman spectra using Gaussian software for the BaTiO3 type perovskite oxides. kindly suggest me.

25 July 2016 3,355 2 View

Info on fluorescence excitation and emission?

Hi, i am working on fluorescence microscopy..i am trying to build a small microscope to study fluorescence (eGFP) ..I would like to know if there is any limitation on the excitation time and...

14 April 2016 1,210 9 View

How do I generate optical delay?

I would like to generate optical delay of around 50 ns. But I dont want to increase the path length a lot, nor can I induce multiple reflections, since that would reduce the intensity of outcoming...

11 September 2015 4,196 18 View

Why is proton stripping reaction more favorable as compare to neutron stripping?

Please explain or suggest where I will get this answer?

20 January 2015 3,739 7 View

Does any one know updated ribosomal databases?

We have used rdp data analysis for ribosomal RNA gene (rDNA) amplicon reads from high-throughput sequencing (sanger sequencing). Almost half of my data was categorized as unclassified. I thought...

11 November 2014 6,977 2 View

How can I construct an extra column in SAP2000 with gridlines?

I am currently modelling a monument. In it I have to construct a extra column and beam, but after providing frame structure the gridline option goes off. So how to construct column after that?

11 June 2014 8,814 3 View

What decides the preference of any basis set used in a method?

Is it true that choosing a basis set is not a science but an art?

20 April 2014 5,385 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View