Has anyone used genomic DNA for Recombinase Polymerase Amplification Primer Screen ?

08 May 2021 0 3K Report

TwistDx the company which sells the TwistDx kits for RPA. It recommends using 40 copies of the target sequence for a primer screen. I understand 40 copies can be made by using Clone. Has anyone tried using the genomic DNA for primer screen?

Badges
Science topic

Similar topics
Chemistry, Organic
Organic

More Vinaykumar Hallur's questions See All

For Plant protein Extraction instead of Liquid N2 can i use Glass beads?

During total Plant Protein extraction commonly use Liquid N2 for removal of moisture from Plant parts, instead of using Liquid N2 can I use Glass beads for protein extraction, is it have any bad...

25 November 2021 8,866 2 View

How can I install Rpy2 in my system with out having the below mentioned errors?

We are currently working on a project which requires us to wrap the r codes into python notebook, for which we preferred rpy2 package. Initially it was working fine, but recently we came across...

25 November 2020 10,034 2 View

Do we need to follow CPCSEA guidelines when using insect models for our research?

Since insects are invertebrates what are the guidelines for handling them in research in India ?

22 September 2018 4,581 2 View

Is there any way of confirming whether a fungus has sporulated without making a tease/tape mount??

I am having a black fungus which is growing very slowly and I am scared that I will contaminate the culture of I open the plate for preparing a tease mount

01 November 2017 7,867 2 View

What has more clinical significance Helicobacter pylori MLST or virulence genotyping ?

I have read that Helicobacter pylori MLST is more meaningful in understanding human migrations rather than helping in understanding the clinical importance of a particular population of...

10 February 2017 3,799 3 View

Why cant we use Bst polymerase(used for LAMP) in place of taq polymerase for PCR ?

I was just thinking why we could not replace the taq polymerase with Bst polymerase used in LAMP and run our PCR ?

28 November 2016 2,744 4 View

Can any one please let me know the informations regarding metal matrix composite of AL 6061+ ZIRCONIUM+ FLY ASH?

i am working on the project of study of machinability of Alluminium 6061, zirconium, fly ash composites. before i take a step ahead i want to get some details about how the results of this...

25 July 2016 9,886 2 View

Join to Mechanical Engineers

I want to join this team

06 April 2011 3,101 1 View

HAs anyone used the new capillary microculture method for culturinh Helicobacter pylori?

Has anyone used the capillary microculture method for isolating Helicobacter pylori from gastric biopsy ? Please have a look at the link below ?

01 January 1970 4,372 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View