TwistDx the company which sells the TwistDx kits for RPA. It recommends using 40 copies of the target sequence for a primer screen. I understand 40 copies can be made by using Clone. Has anyone tried using the genomic DNA for primer screen?
We are currently working on a project which requires us to wrap the r codes into python notebook, for which we preferred rpy2 package. Initially it was working fine, but recently we came across...
25 November 2020 9,871 2 View
Since insects are invertebrates what are the guidelines for handling them in research in India ?
22 September 2018 4,362 2 View
I am having a black fungus which is growing very slowly and I am scared that I will contaminate the culture of I open the plate for preparing a tease mount
01 November 2017 7,733 2 View
I was just thinking why we could not replace the taq polymerase with Bst polymerase used in LAMP and run our PCR ?
28 November 2016 2,605 4 View
i am working on the project of study of machinability of Alluminium 6061, zirconium, fly ash composites. before i take a step ahead i want to get some details about how the results of this...
25 July 2016 9,734 2 View
I want to join this team
06 April 2011 2,959 1 View
Has anyone used the capillary microculture method for isolating Helicobacter pylori from gastric biopsy ? Please have a look at the link below ?
01 January 1970 4,201 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...
03 March 2021 8,066 4 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...
01 March 2021 360 7 View
I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...
28 February 2021 606 3 View
hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?
28 February 2021 1,254 3 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View