Is there any way of confirming whether a fungus has sporulated without making a tease/tape mount??

More Vinaykumar Hallur's questions See All

For Plant protein Extraction instead of Liquid N2 can i use Glass beads?

During total Plant Protein extraction commonly use Liquid N2 for removal of moisture from Plant parts, instead of using Liquid N2 can I use Glass beads for protein extraction, is it have any bad...

25 November 2021 8,866 2 View

Has anyone used genomic DNA for Recombinase Polymerase Amplification Primer Screen ?

TwistDx the company which sells the TwistDx kits for RPA. It recommends using 40 copies of the target sequence for a primer screen. I understand 40 copies can be made by using Clone. Has anyone...

07 May 2021 2,631 0 View

How can I install Rpy2 in my system with out having the below mentioned errors?

We are currently working on a project which requires us to wrap the r codes into python notebook, for which we preferred rpy2 package. Initially it was working fine, but recently we came across...

25 November 2020 10,034 2 View

Do we need to follow CPCSEA guidelines when using insect models for our research?

Since insects are invertebrates what are the guidelines for handling them in research in India ?

22 September 2018 4,581 2 View

What has more clinical significance Helicobacter pylori MLST or virulence genotyping ?

I have read that Helicobacter pylori MLST is more meaningful in understanding human migrations rather than helping in understanding the clinical importance of a particular population of...

10 February 2017 3,799 3 View

Why cant we use Bst polymerase(used for LAMP) in place of taq polymerase for PCR ?

I was just thinking why we could not replace the taq polymerase with Bst polymerase used in LAMP and run our PCR ?

28 November 2016 2,744 4 View

Can any one please let me know the informations regarding metal matrix composite of AL 6061+ ZIRCONIUM+ FLY ASH?

i am working on the project of study of machinability of Alluminium 6061, zirconium, fly ash composites. before i take a step ahead i want to get some details about how the results of this...

25 July 2016 9,886 2 View

Join to Mechanical Engineers

I want to join this team

06 April 2011 3,101 1 View

HAs anyone used the new capillary microculture method for culturinh Helicobacter pylori?

Has anyone used the capillary microculture method for isolating Helicobacter pylori from gastric biopsy ? Please have a look at the link below ?

01 January 1970 4,372 3 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

04 August 2024 5,228 4 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Publishing a checklist?

Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...

01 August 2024 6,134 2 View

How can parents ensure their drinking water is free from harmful contaminants?

27 July 2024 8,722 6 View

HEC 1A & HEC1B Cell Lines?

Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...

20 July 2024 4,143 2 View

Masonry Interaction Module ABAQUS?

I am simulating interlocking / dry stacked masonry in Abaqus. I am using a friction coefficient of 0.6 for my model as it is stated in the previous research as well as in the base paper. The...

16 July 2024 5,047 0 View

How can we simulate(with FEM) the shape of Mobius strip using Abaqus Learning edition?

Aim is to simulate the shape of Mobius strip (an elastic ribbon) in simple way ( using Abaqus Learning edition). In literature, Mobius strip is modeled as Kirchhoff Rod / Cosserat plate. But, such...

15 July 2024 4,388 1 View