How can I install Rpy2 in my system with out having the below mentioned errors?

We are currently working on a project which requires us to wrap the r codes into python notebook, for which we preferred rpy2 package. Initially it was working fine, but recently we came across...

25 November 2020 9,871 2 View

Do we need to follow CPCSEA guidelines when using insect models for our research?

Since insects are invertebrates what are the guidelines for handling them in research in India ?

22 September 2018 4,362 2 View

Why cant we use Bst polymerase(used for LAMP) in place of taq polymerase for PCR ?

I was just thinking why we could not replace the taq polymerase with Bst polymerase used in LAMP and run our PCR ?

28 November 2016 2,605 4 View

Can any one please let me know the informations regarding metal matrix composite of AL 6061+ ZIRCONIUM+ FLY ASH?

i am working on the project of study of machinability of Alluminium 6061, zirconium, fly ash composites. before i take a step ahead i want to get some details about how the results of this...

25 July 2016 9,734 2 View

Join to Mechanical Engineers

I want to join this team

06 April 2011 2,959 1 View

HAs anyone used the new capillary microculture method for culturinh Helicobacter pylori?

Has anyone used the capillary microculture method for isolating Helicobacter pylori from gastric biopsy ? Please have a look at the link below ?

01 January 1970 4,201 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View