Criteria to consider when choosing format of cell lines in terms of ease of handling and revivability. Pros and cons of frozen culture in cryo vials and growing culture in culture flask?
I have not bought it in a culture flask before, so I do not know the pros and cons of that. But our institute buys cell lines in frozen cultures in cryovials all the time. There was an incident when the arrival of the cell lines at the institute was not informed in advanced, and no experts were around - that the research interns simply kept the cell lines at -20'C freezer! Of course, those cell lines did not survive. Anyway, the moral of the story is, if the cell lines in cryovials come, it is best to keep them straight in liquid nitrogen if no expert is around.
Like Nural I've never purchased in a culture flask before. I can recommend cryovials, assuming they are stored properly on arrival. We store ours in a -80 freezer and attain viabilities of >90% when the cells are preserved in cryostore.
- Badges
- Science topic