Hello!!
I performed a conversion using one DNA with 200 ng (calculated) concentration and add 130 ul CT Reagent of EZ Methylation DNA Gold (Zymo). The result of this reaction was complete conversion! But, when I tried with 8-10 sample did not work! What I can do now? I came back for a conversion experiment with 2 sample or when I add the CT reagent, I need to protect the reaction of light?
Best regards,
Thatiana
I bought this year this kit and used the same one to treatment my sample. I will contact Zymo about my problems. Thank you for share your experience! T.
Not sure if this got solved for you but what was the DNA quality like from the extractions and how do you know there was complete conversion? Was this from a follow on qMSP and could it be that the region you are looking at isnt methylated in all your samples?
Hopefully this got sorted for you though :)
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