The C-terminus also known as the carboxyl-terminus, terminated by a free carboxyl group (-COOH). The convention for writing peptide sequences is to put the C-terminal end on the right and write the sequence from N- to C-terminus. :) refer to pic. A tetrapeptide (example: Val-Gly-Ser-Ala) with green highlighted N-terminal α-amino acid (example: L-valine) and blue marked C-terminal α-amino acid (example: L-alanine)
as you can see from 1 to k37 is signal and between k37 and k111 is N terminal and then C terminal. how i can now from which amino acid in my protein c terminal start. even i use protease enzyme i can not now that also from Multiple sequence alignment