Wafa Ali Eltayb

4 Questions 5 Answers 0 Followers

Questions related from Wafa Ali Eltayb

What is the proper method that can help to improve crystal diffraction from 5 Angstrom to 1.2 ?

is there is any method i could follow to get high diffraction resolution rather than dehydration? thanks in advance :)

01 January 2016 9,953 4 View

Is there any suggestion to avoid protein aggregation during purification?

my protein belong to glycosyltransferase family 2 the catalytic domain adopt GT_A type fold , i used to purify with 20mM tris 8.0 100mM Nacl without additive, though the protein pI is 4.8. the...

12 December 2015 5,260 8 View

Why do protein crystal disappeared after 48 hour?

after setup crystallization screening, crystal were grown overnight in needle form. at this condition 5% Tacsimate 7.0 0.1M HEPES 7.0 10% PEG5K MME, but after 48 hours were disappeared, i have...

12 December 2015 3,174 3 View

Hi every one , can any one suggest me proper way to improve protein crystal size and diffraction ??

i have optimize different pH at pH blow 7.0 crystal was in needle cluster form at pH above 7.0 crystal has octagonal shape but it's tiny ..? thanks in advance

06 June 2015 1,848 3 View