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my protein is glycosyltransferase pi 5.2 i done crystal whit all this buffer i can not find any crystal. when i do crystal i use UDP and UDP+DDTno aggregation when i purify. i can see that...
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when i have sequence of the protein how can i know the c terminal and n terminal start from which amino acid
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when we do transformation by two plasmid, bacteria can express two of them or it chose one. i want to know that depend on what
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