my protein is glycosyltransferase pi 5.2 i done crystal whit all this buffer i can not find any crystal. when i do crystal i use UDP and UDP+DDTno aggregation when i purify. i can see that protein precipitant just when i put it in crystal solution even i use 2mg for crystals scrying . i to need to add Imidazole to protein purification buffer or no to get a crystal ? i try to delete the link between N domin and C domin but can not find protein at all
30mM NaCL 70mMTris HCL 10mM glucose pH8
100mM NaCL 20mMTris HCL 10mM glucose pH8
1M NaCL 20mMTris HCL 10mM glucose pH8+ 2mM EDTA
250mM NaCL 20mMTris HCL 8%glycerol pH8
250mM NaCL 20mM HEPES HCL 2%glycerol pH7
You will probably need to try more than five conditions.
You can try PEG, ammonium sulphate. There are plenty of additives.
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