I am going to prepare a culture medium for the buds of a calla lily, but I'm not too sure If I have to add the antibiotics after the sterilization or before.
Can anybody help me?
Thank you =)
Thank you Ravin
Do you know how can i sterilized the solution of antibiotics, to ensure no contamination? and also a technique to pour the solution of antibiotics?
=P
Basically antibiotic sterilization is not required, but 0.2 micron filtration could be used and make a well on sterile agar plate by puncturing the gel with sterilized cork borer......
I am interested in this question, because i want to culture anaerobs in blood gelose and i know I should add antibiotics. Would you give me the protocole of preparation of sheep blood agar with antibiotics?
I am also interested in this question, i have to make a viable count for resistant colonies that will generate when using antibiotics mixed with the agar media!
So, ow can i mix the antibiotic with the agar media, can i mix it before pouring the agar to solidify? and this test will be called viable count or agar well diffusion method (if i make a hole in the agar to add the antibiotics)?
Antibiotics properties are affected by high temperature, so adding antibiotics after sterilization and before the agar solidified is the best.
good luck .................regards
Because the antibiotic is sensitive to high temperatures, antibiotics should be added after sterilization of the culture medium. Just be careful:
1. It's time to add antibiotics before the agar solidified.
2. Add antibiotics to the culture medium with a 0.2-micron filter.
Best Regards,
Usually, antibiotics are added for sterilization by two ways. Before entering explants to laminar hode ( by soaking explants in it for appropriate time) or by addition to the culture medium after autoclaving ( by filteration). Good luck!
Apply antibiotic after filter sterilization technique and when the media gets cool, just before solidification.
I have another question in this area, I want to know how long I can keep agar media containing antibiotics in 4'C?
This very good question. antibiotics there are two types of antibiotics the first one is thermolabile and second one is autoclvable. use the second one is better and I advice you to use tetracycline and rifampicin with a rate 10-20 mg/L. up to 3 subculture
Due to the heat sensitivity of antibiotics, the best way to add them is after autoclaving.
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