Could someone please suggest a suitable program for assembling plasmids containing repetitive sequences from short paired-end reads?

Hello! I'm trying to assemble some plasmid vectors that contain two repetitive regions (with around 500 and 250 bp) and it is being hard to get the complete sequence. If someone knows a better...

03 February 2021 6,379 3 View

How to calculate the number of degrees of freedom in a Mantel test?

Hello, I've performed a Mantel test between my microbiological data and the geographic distance where those samples were collected, using a table with 516 samples. However, the test doesn't...

16 January 2021 8,713 4 View

Which are the Non Small Cell Lung Cancer markers?

I would like to sort from a fresh specimen of lung cancer Non-Small Lung Cancer cells and cancer-associated fibroblasts to establish two different cell cultures. Which markers should I use? Thank...

04 January 2021 7,986 3 View

Women learning styles/ Women cognition development- Any suggestions for research based on Belenky's work?

I'm looking to expand my sources on the topic

10 December 2020 5,426 17 View

Sankey Diagram: Waht is the sample size needed?

Hello everyone. I would like to ask your help on the decision about the best methodology to determine the sample size to conduct an observational study. This is a study to be carried out by a...

16 September 2020 9,014 1 View

How to convert, excel files into (.vgd) or (.vms), ( .dts), (.dpr), (.vgl) files ?. Is there any software is available for this conversion?

I need a compatible file with Avantage for XPS of Themo Scientific but y only have excel files (.xls)

07 September 2020 215 3 View

Any expert on air pollution and its consequences on soil, plants and food production that is available to answer some questions?

Hello, I am a 3rd year Journalism student at Solent University. I am currently working on my Final Major Project. My project is about how air pollution influences our environment, and I will...

16 February 2020 6,332 2 View

I don't get a synthesis sample by pcr, what could I modify? considering that I already started from a standard protocol, I still don't get samples?

am carrying out a final point pcr from a standardized protocol, but I don't get results. I have modified concentrations of magnesium dichloride, DNTPS, DMSO, change the reaction water, the quality...

06 November 2019 7,767 2 View

In the sphere of education for the third millennium could education and inclusive school be an answer?

An inclusive education implies the development of inclusive societies. The inclusive, open-minded school for all educators and everyone, flexible and anchored in solving any educational problem...

07 March 2019 5,999 6 View

How to read the results from Illumina MiSeq machine?

Hi, everyone, I sequenced some samples in the Illumina MiSeq, got the results but now I don't know how to analyze them. I already read the User guide from Illumina, but it's only about the...

02 December 2018 9,374 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View