I am working with size distributions of nanoparticles. But I also need to show my results as a function of area (#NP/area). Does anybody know how calculate the area of a TEM micrograph?
If you have a scale bar, then simply measure the length of the scale bar lb in pixel (px), the length of the image li in pixel and note the scalebar legend f in (n)m
The area will be:
li^2 /lb^2 * f^2 (in units of f^2)
If you are not sure if the scalebar is correct you can use reference sample for calibration.