Parastou Rahimizadeh

9 Questions 24 Answers 0 Followers

Questions related from Parastou Rahimizadeh

Why sequencing result doesn't show any homology with my expected gene sequence?

I had a BL21 (DE3) cell which I always use to express GFP protein which fused to a coil. Based on the vector map given to me, this gene is inside the pQE80 vector which has resistance to...

15 December 2020 4,489 4 View

Why the expressed protein band Molecular Weight (MW) is lower than expectation?

I tried to express my protein in BL21(DE3) bacteria. The desired protein MW is 65.5 KDa and I know my protein will be in an insoluble form (inclusion body). I separate soluble and insoluble...

21 October 2020 4,061 8 View

How can i interpret ITC result?

I want to investigate the interaction between two proteins by ITC. I choose two proteins that I'm sure don't interact with each other as a control. but ITC results show there is some interaction...

23 June 2020 2,331 5 View

How can one reduce As+3 to As0 without interference of As+5?

When we have two forms of As in an environment, how and in which potential does the reduction of As+3 to As0 occur, and the interference of As+5 form is zero?

16 February 2015 673 3 View

Can anyone help me with FTIR interpretation?

I immobilize enzyme on chitosan macrobead with Glutaraldehyde as crosslinker. I want to know how FTIR confirm immobilization? Which wavelength is related to chitosan with glutaraldeyde and which...

06 January 2015 4,196 9 View

Why optimum pH shift from 6.5 to 8 after immobilization?

I immobilize enzyme on chitosan macrobead after activating them with glutaraldehyde. I want to set up optimum condition for emobilized enzyme. Optimum pH shift from6.5 to 8?! Is there any reason...

02 January 2015 664 6 View

Why I can't immobilize enzyme on chitosan macrobead?

I want to immobilize my produced enzyme on chitosan macrobead with glutaraldehyde as crosslinker.i treat 1 gram of chitosan macrobead by10ml glutaraldehyde 0.3% based on literature,after that...

28 July 2014 2,222 12 View

What is my problem in cloning?

I want to clone my gene to expreesion vector in Ecoli, after I clean my pcr product, I have a good band. In digestion step, after digesting the gene I don't get any band, but I perform a ligation...

01 February 2014 9,732 16 View

Gel mobility shift on SDS-PAGE

I tried to express a protein in the bacteria system (BL21 (DE3)). This expressed protein in any temperature and condition shows a bond lower than expectation on SDS-PAGE (theoretical MW:66284 Da)....

01 January 1970 4,714 3 View