After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How to use -dhdl flag during mdrun with gromacs?

I am trying to use the gmx energy model to make free energy calculations upon binding of a ligand to a receptor. To do this I am invoking the energy module and adding the -odh flag: gmx energy -f...

09 November 2020 1,631 3 View

Preparation of mouse thymus for Flow Cytometry?

Hi, I'm trying to develop a protocol for prepping thymus tissues in mice. We will be using NSG mice in our lab and the goal is to get good consistent single cell suspensions to stain for FACS....

26 October 2020 10,006 3 View

Issues generating hbmap.xpm file from gromacs hbond module, using -xpm flag?

I am currently trying to monitor the changes that a ligand elicits in the hydrogen bonding pattern of a protein during binding. I can easily see with the -num flag that the number of internal...

18 August 2020 7,973 1 View

How to publish my old paper in an open journal?

Dear Research Gate Community, I'm finally able to publish my dissertation paper and I'm keen to find the right place to publish it. My old university has just given me permission to publish it...

13 July 2020 1,938 3 View

Recruitment of Children - Ages 5 - 12 year olds - best methods?

Hi All, I am running a study which requires two visits for children to complete an EEG (Brain scan) and an eye tracking task. We are currently working a recruitment plan for after the Covid-19...

30 March 2020 8,737 3 View

Which methods do academic writing and literacy facilitators apply to incorporate students' primary discourses into learning experiences?

Greetings Colleagues Primary discourses are those ways of using language, literacy and multiple modes of communicating as acquired in our homes, among our families and in our initial schooling...

28 December 2019 6,472 5 View

Is social media a good platform that will bring change in elections?

South African elections have ushered in a new thinking on the role of social media in influencing elections. The EFF had the highest number of young people who where on social media. ( 6 million)...

12 May 2019 4,031 6 View

How much solvent (ethanol) would I need to extract capsaicin (and other capsaicinoids) from siling haba?

Siling haba is a variation of Capsicum annuum var longum. The capsaicin extracted will be tested against aphids to test its insecticidal properties. Someone suggested that I should use 5ml/g or 2g...

12 February 2019 1,373 3 View

FMRI power analysis for RSA (representational similarity analysis)?

Dear community, I'm looking into ways how to do an a-priori power analysis for an fMRI experiment where the main analysis will be a representational similarity analysis (RSA). The experiment...

06 February 2019 1,338 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View