Oliver Mikse

10 Questions 5 Answers 0 Followers

Questions related from Oliver Mikse

What are some CROs that can do PK with wet lab accessability?

I'm looking for some CROs that can do the PK analysis from mouse plasma that we already have. We need to know the concentration of our compound in the plasma so I assume that would require some...

27 July 2023 2,656 0 View

Are Calu-6 not really NSCLC cells?

I've been doing research and noticing that Calu-6 aren't exactly considered to be of lung tissue origin anymore. The ATCC has them as designation "unknown" and some list it as "probanly...

22 August 2022 3,807 0 View

Are there recommended workshops to develop math skills?

Hello, I have a Research Associate (tech) who is wonderful and successful in many ways but their math skills need improvement. They struggle with the math skills required to make dilutions and...

02 February 2022 6,742 0 View

Preparation of mouse thymus for Flow Cytometry?

Hi, I'm trying to develop a protocol for prepping thymus tissues in mice. We will be using NSG mice in our lab and the goal is to get good consistent single cell suspensions to stain for FACS....

27 October 2020 10,232 3 View

Difference in onset time for diabetes model rip-mOVA male vs female mice?

Hey everyone. We're planning on doing a diabetes study with the rip-mOVA mouse model ( https://www.jax.org/strain/005431 ) and are wondering if anyone has experience with this model and are there...

28 October 2019 3,517 0 View

Alternatives to NK-92 cells for ADCC assay?

Hello everyone. I am trying to find alternatives to NK-92 cells for our ADCC assay. I know of KHYG-1 and the KILR1 CD16 line. Any others that have proven successful in your assays?

29 April 2019 8,960 0 View

What can I use as a positive reporter for transfections?

I'm trying to transfect CHO (and other cells later on) with a new construct. I've had success in the past with Lipofectamine on lung cancer cell lines and will probably try the same. I'm wondering...

21 March 2018 339 5 View

How soon does B-Catenin get degraded by siRNA?

Hi everyone. How soon after targeting b-Catenin with siRNA can we start to expect degradation of the RNA? Hours? Minutes? Trying to find answers for both in vitro and in vivo (xenograft) cancer...

11 May 2017 1,514 3 View

What is the best human gastric cancer cell line to use for xenografts?

Hello,I'm trying to get some mouse xenografts started for a gastric model that basically involves efficacy testing. What are some good human gastric cancer xenografts that give consistent tumors...

18 January 2017 708 6 View

What's the best liver xenograft mouse model?

I'm having trouble with liver cancer cell lines for subQ injection in mouse xenograft. I've been using HepG2, Hep3B, and Huh7 and although I do get some tumors forming after 3 weeks or so, the...

03 August 2016 8,626 6 View