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Questions related from Oliver Mikse
Hello, I have a Research Associate (tech) who is wonderful and successful in many ways but their math skills need improvement. They struggle with the math skills required to make dilutions and...
02 February 2022 6,699 0 View
Hi, I'm trying to develop a protocol for prepping thymus tissues in mice. We will be using NSG mice in our lab and the goal is to get good consistent single cell suspensions to stain for FACS....
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Hello everyone. I am trying to find alternatives to NK-92 cells for our ADCC assay. I know of KHYG-1 and the KILR1 CD16 line. Any others that have proven successful in your assays?
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I'm trying to transfect CHO (and other cells later on) with a new construct. I've had success in the past with Lipofectamine on lung cancer cell lines and will probably try the same. I'm wondering...
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Hi everyone. How soon after targeting b-Catenin with siRNA can we start to expect degradation of the RNA? Hours? Minutes? Trying to find answers for both in vitro and in vivo (xenograft) cancer...
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Hello,I'm trying to get some mouse xenografts started for a gastric model that basically involves efficacy testing. What are some good human gastric cancer xenografts that give consistent tumors...
18 January 2017 656 6 View
I'm having trouble with liver cancer cell lines for subQ injection in mouse xenograft. I've been using HepG2, Hep3B, and Huh7 and although I do get some tumors forming after 3 weeks or so, the...
03 August 2016 8,589 6 View
