Oliver Mikse

5 Questions 5 Answers 0 Followers

Questions related from Oliver Mikse

Preparation of mouse thymus for Flow Cytometry?

Hi, I'm trying to develop a protocol for prepping thymus tissues in mice. We will be using NSG mice in our lab and the goal is to get good consistent single cell suspensions to stain for FACS....

27 October 2020 10,117 3 View

What can I use as a positive reporter for transfections?

I'm trying to transfect CHO (and other cells later on) with a new construct. I've had success in the past with Lipofectamine on lung cancer cell lines and will probably try the same. I'm wondering...

21 March 2018 287 5 View

How soon does B-Catenin get degraded by siRNA?

Hi everyone. How soon after targeting b-Catenin with siRNA can we start to expect degradation of the RNA? Hours? Minutes? Trying to find answers for both in vitro and in vivo (xenograft) cancer...

11 May 2017 1,461 3 View

What is the best human gastric cancer cell line to use for xenografts?

Hello,I'm trying to get some mouse xenografts started for a gastric model that basically involves efficacy testing. What are some good human gastric cancer xenografts that give consistent tumors...

18 January 2017 642 6 View

What's the best liver xenograft mouse model?

I'm having trouble with liver cancer cell lines for subQ injection in mouse xenograft. I've been using HepG2, Hep3B, and Huh7 and although I do get some tumors forming after 3 weeks or so, the...

03 August 2016 8,580 6 View