Why higher OD in no substrate blank than inactivated enzyme blank?

Hi all, I am doing an Alkaline Phosphatase activity assay using PPi as substrate. I am using PiBlue Kit to measure amount of Pi generated at 620nm. I am getting high OD values in my no substrate...

08 September 2020 8,708 6 View

Active Response rate?

18 August 2019 5,112 2 View

How did you ensure the normality of data? What statistical tests you run?

Normality of Data

18 August 2019 2,208 3 View

What are the preconditions or steps or regression, mediation and moderation of Macros Process/Andrew A. f. Hayes Process?

Pre Conditions of MACROS PROCESS DEVELOPED BY ANDREW a f HAYES

18 August 2019 7,863 4 View

Visualizing cell pellet after FACS?

Hi all, I am sorting small number of cells (10,000-50,000)in 1.5 ml tubes using FACS. I am unable to see pellet of cells in the tube. I understand that it is difficult to observe pellet with such...

25 February 2018 2,084 6 View

Saffron micropropagation (need help) ?

I have worked micropropagation of saffron. I have a problem. Saffron bulbs explant growth a few day in first week on MS media then stop growth. After no growth no die. Stay stable. I tried all PGR...

04 August 2016 4,969 5 View

What can be Lineage-CD45+SCA-1+ positive Cells in brain?

Hi all, I am working on innate lymphoid cells and found that in our settings we have significant Lin-CD45+SCA-1+ cells. I further analyzed these cells for the expression of THY1, KLRG1, IL7ra and...

26 July 2016 908 4 View

Mice deficient in ILC3?

Dear all, Are there any mice strain available which are deficient specifically in group 3 or group 2 ILCs? best, Arshed

26 April 2016 5,313 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View