What's the difference between splenic (mouse) dendritic cells and macrophages?

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Anton Beletskii

Seems that most of the answers can be found in this open access article:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3987771/#SD2

Most of the relevant info is in Supplementary Materials.

Kottarappat N Dileepan

Please see the article by Ziegler-Heitbrock et al, "Nomenclature of monocytes and dendritic cells in blood", Blood 116:e74-e80, 2010

Brett P Gross

There are many phenotypes in the literature, but for basic studies involving murine splenocytes you can typically use MHCII+CD11b+CD11c-/lowF4/80+ for macrophages and MHCII+CD11b-/+CD11chiF4/80- for DCs.