Arshed Nazmi

5 Questions 13 Answers 0 Followers

Questions related from Arshed Nazmi

Why higher OD in no substrate blank than inactivated enzyme blank?

Hi all, I am doing an Alkaline Phosphatase activity assay using PPi as substrate. I am using PiBlue Kit to measure amount of Pi generated at 620nm. I am getting high OD values in my no substrate...

09 September 2020 8,862 6 View

Visualizing cell pellet after FACS?

Hi all, I am sorting small number of cells (10,000-50,000)in 1.5 ml tubes using FACS. I am unable to see pellet of cells in the tube. I understand that it is difficult to observe pellet with such...

26 February 2018 2,178 6 View

What can be Lineage-CD45+SCA-1+ positive Cells in brain?

Hi all, I am working on innate lymphoid cells and found that in our settings we have significant Lin-CD45+SCA-1+ cells. I further analyzed these cells for the expression of THY1, KLRG1, IL7ra and...

27 July 2016 1,008 4 View

Mice deficient in ILC3?

Dear all, Are there any mice strain available which are deficient specifically in group 3 or group 2 ILCs? best, Arshed

27 April 2016 5,404 3 View

What's the difference between splenic (mouse) dendritic cells and macrophages?

Dear All, Is there any distinct markers to differentiate macrophages and dendritic cells from mouse spleen?

01 December 2015 9,469 3 View